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Invitrogen
Phospho-GLI2 (Ser136) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-GLI2 (Ser136) Antibody (PA5-105348) in WB
Western blot analysis of Phospho-GLI2 (Ser136) in extracts from MC-3T3-E1 cells (H2O2 treatment). Samples were incubated with Phospho-GLI2 (Ser136) polyclonal antibody (Product # PA5-105348). The lane on the left was treated with blocking peptide.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105348
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GLI2(Accession P10070), corresponding to amino acid residues around phosphorylated Ser136.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Gli2 only when phosphorylated at Ser136.
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; oncogene GLI2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; transcription factor; unnamed protein product; zinc finger protein GLI2
Gene Aliases: CJS; HPE9; PHS2; THP1; THP2
UniProt ID: (Human) P10070, (Mouse) Q0VGT2
Entrez Gene ID: (Human) 2736, (Mouse) 14633
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
transcription regulatory region sequence-specific DNA binding
chromatin binding
chromatin DNA binding
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
osteoblast differentiation
kidney development
osteoblast development
regulation of transcription from RNA polymerase II promoter
smoothened signaling pathway
pattern specification process
axon guidance
ventral midline development
hindgut morphogenesis
heart development
anatomical structure morphogenesis
epidermal cell differentiation
proximal/distal pattern formation
regulation of gene expression
floor plate formation
spinal cord dorsal/ventral patterning
ventral spinal cord development
cerebellar cortex morphogenesis
spinal cord ventral commissure morphogenesis
pituitary gland development
lung development
mammary gland development
hindbrain development
hair follicle morphogenesis
positive regulation of T cell differentiation in thymus
tube development
odontogenesis of dentin-containing tooth
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryonic digestive tract development
developmental growth
neuron development
branching morphogenesis of an epithelial tube
cellular response to virus
in utero embryonic development
morphogenesis of an epithelium
chondrocyte differentiation
regulation of transcription, DNA-templated
positive regulation of cell proliferation
regulation of smoothened signaling pathway
anterior/posterior pattern specification
dorsal/ventral pattern formation
gene expression
spinal cord motor neuron differentiation
smoothened signaling pathway involved in spinal cord motor neuron cell fate specification
dorsal/ventral neural tube patterning
neural tube development
cell differentiation
forebrain development
negative regulation of chondrocyte differentiation
positive regulation of collagen biosynthetic process
embryonic digit morphogenesis
negative regulation of apoptotic process
positive regulation of neuron differentiation
negative regulation of smoothened signaling pathway
embryonic organ morphogenesis
anatomical structure formation involved in morphogenesis
gland development
notochord regression
head development
prostatic bud formation
mammary gland duct morphogenesis
cochlea morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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