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Invitrogen

Phospho-RIP2 (Ser531) Polyclonal Antibody

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Phospho-RIP2 (Ser531) Polyclonal Antibody

Product Details

PA5-105392

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RIPK2(Accession O43353), corresponding to amino acid residues around phosphorylated Ser531.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816820

Product Specific Information

Antibody detects endogenous levels of RIPK2 only when phosphorylated at Ser531.

Target Information

RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; EC 2.7.11.1; growth-in; kinase RIPK2; receptor (TNFRSF)-interacting serine-threonine kinase 2; Receptor-interacting protein 2; Receptor-interacting serine/threonine-protein kinase 2; RIP 2; RIP-2; RIP-like; RIP-like-interacting CLARP kinase; Tyrosine-protein kinase RIPK2; WUGSC:H_RG437L15.1

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Gene Aliases: 2210420D18Rik; CARD3; CARDIAK; CCK; D4Bwg0615e; RICK; RIP2; RIPK2; UNQ277/PRO314/PRO34092

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UniProt ID: (Mouse) P58801

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Entrez Gene ID: (Rat) 362491, (Mouse) 192656

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Function(s)
protein serine/threonine kinase activity ATP binding LIM domain binding protein homodimerization activity CARD domain binding nucleotide binding protein kinase activity non-membrane spanning protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
positive regulation of cytokine-mediated signaling pathway adaptive immune response positive regulation of T-helper 1 type immune response protein phosphorylation I-kappaB kinase/NF-kappaB signaling positive regulation of peptidyl-threonine phosphorylation positive regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of stress-activated MAPK cascade positive regulation of immature T cell proliferation positive regulation of peptidyl-serine phosphorylation toll-like receptor 2 signaling pathway toll-like receptor 4 signaling pathway T cell proliferation positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of cysteine-type endopeptidase activity involved in apoptotic process response to exogenous dsRNA innate immune response positive regulation of T-helper 1 cell differentiation positive regulation of JNK cascade positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-positive bacterium T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity positive regulation of ERK1 and ERK2 cascade nucleotide-binding oligomerization domain containing 1 signaling pathway nucleotide-binding oligomerization domain containing 2 signaling pathway response to interleukin-1 response to interleukin-12 response to interleukin-18 cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to muramyl dipeptide immune system process apoptotic process phosphorylation regulation of apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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