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Invitrogen
Phospho-L-Plastin (Tyr300) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-L-Plastin (Tyr300) Antibody (PA5-105493) in ICC/IF
Immunocytochemistry analysis of Phospho-L-Plastin (Tyr300) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-L-Plastin (Tyr300) polyclonal antibody (Product # PA5-105493) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjug... View More
Product Details
PA5-105493
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human LCP1(Accession P13796), corresponding to amino acid residues around phosphorylated Tyr300.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Plastin L only when phosphorylated at Tyr300.
Target Information
L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosterones in androgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa macrophage protein; bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin)); DKFZp781A23186; epididymis secretory protein Li 37; FLJ25423; FLJ26114; FLJ39956; L-fimbrin; L-plastin; L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (65 kDa macrophage protein) (PP65); L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P); LC64P; LCP-1; Lymphocyte cytosolic protein 1; Lymphocyte cytosolic protein-1 (plasmin); plastin 2, L; Plastin-2; pp65; unnamed protein product
Gene Aliases: AW536232; CP64; D14Ertd310e; HEL-S-37; L-PLASTIN; LC64P; LCP-1; LCP1; LPL; PLS2; pp65
UniProt ID: (Human) P13796, (Mouse) Q61233
Entrez Gene ID: (Human) 3936, (Mouse) 18826
T cell activation involved in immune response
protein kinase A signaling
cell migration
extracellular matrix disassembly
cortical actin cytoskeleton organization
organ regeneration
regulation of intracellular protein transport
wound healing, spreading of cells
actin filament bundle assembly
actin filament network formation
actin crosslink formation
positive regulation of podosome assembly
response to wounding
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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