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Invitrogen
Phospho-Beclin 1 (Ser90, Ser93, Ser96) Polyclonal Antibody
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FIGURE: 1 / 6
Phospho-Beclin 1 (Ser90, Ser93, Ser96) Antibody (PA5-105647) in ICC/IF
Immunocytochemistry analysis of Phospho-Beclin 1 (Ser90, Ser93, Ser96) in HepG2 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-Beclin 1 (Ser90, Ser93, Ser96) polyclonal antibody (Product # PA5-105647) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-105647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BECN1(Accession Q14457), corresponding to amino acid residues around phosphorylated Ser90.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of Beclin 1 only when phosphorylated at Ser90/93/96.
Target Information
Beclin 1 plays a central role in autophagy. It acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate. Different PI3K complex forms are believed to play a role in multiple membrane trafficking pathways - PI3KC3-C1 is involved in the initation of autophagosomes and PI3KC3-C2 is involved in the maturation of autophagosomes and endocytosis. Beclin 1 also plays a role in antiviral host defense.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG6; Bcl-2-interacting protein beclin; beclin 1 (coiled-coil, myosin-like BCL2-interacting protein); Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; coiled-coil, myosin-like BCL2-interacting protein; Protein GT197
Gene Aliases: 4921513J16Rik; 5430417M23Rik; Atg6; BECN1; GT197
UniProt ID: (Rat) Q91XJ1, (Mouse) O88597
Entrez Gene ID: (Rat) 114558, (Mouse) 56208
autophagosome assembly
mitophagy
response to hypoxia
autophagy
cellular response to nitrogen starvation
lysosome organization
negative regulation of cell proliferation
positive regulation of autophagy
positive regulation of macroautophagy
CVT pathway
response to vitamin E
response to drug
nucleophagy
late endosome to vacuole transport
neuron development
beta-amyloid metabolic process
regulation of catalytic activity
defense response to virus
response to other organism
negative regulation of cell death
cellular response to aluminum ion
cellular response to epidermal growth factor stimulus
positive regulation of mitophagy
cytokinesis
angiogenesis
cell cycle
mitotic metaphase plate congression
macroautophagy
regulation of cytokinesis
receptor catabolic process
cellular response to glucose starvation
negative regulation of apoptotic process
cell division
mitophagy in response to mitochondrial depolarization
positive regulation of attachment of mitotic spindle microtubules to kinetochore
negative regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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