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Invitrogen
Phospho-ZAK (Ser165) Polyclonal Antibody
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Phospho-ZAK (Ser165) Antibody (PA5-105924) in ICC/IF
Immunocytochemistry analysis of Phospho-ZAK (Ser165) in A549 cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-ZAK (Ser165) polyclonal antibody (Product # PA5-105924) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-105924
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human MAP3K20(Accession Q9NYL2), corresponding to amino acid residues around phosphorylated Ser165.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of ZAK only when phosphorylated at Ser165.
靶标信息
ZAK is a leucine zipper-containing mitogen-activated protein related to the MLKs in both sequence and function. Like the MLKs and other MAP3Ks, ZAK activates MAP2Ks. ZAK is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif. This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HCCS-4; Human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif kinase ZAK; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen-activated protein kinase kinase kinase 20; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; Mixed lineage kinase-related kinase; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MRK; sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; sterile-alpha motif and leucine zipper containing kinase AZK
基因别名: AV006891; B230120H23Rik; HCCS4; MAP3K20; MLK7; MLTK; MLTKalpha; MLTKbeta; RGD1561394; ZAK
UniProt ID: (Mouse) Q9ESL4
Entrez Gene ID: (Mouse) 65964, (Rat) 311743
nucleotide binding
magnesium ion binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
poly(A) RNA binding
metal ion binding
immunoglobulin
non-receptor serine/threonine protein kinase
cell cycle checkpoint
DNA damage checkpoint
MAPK cascade
activation of MAPKK activity
protein phosphorylation
cytoskeleton organization
cell cycle
cell cycle arrest
activation of JUN kinase activity
response to radiation
phosphorylation
intracellular signal transduction
positive regulation of apoptotic process
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