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Invitrogen
Phospho-PDHA1/PDHA2 (Ser293, Ser291) Polyclonal Antibody
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FIGURE: 1 / 9
Phospho-PDHA1/PDHA2 (Ser293, Ser291) Antibody (PA5-106200) in ICC/IF
Immunofluorescent analysis of Phospho-PDHA1/PDHA2 (Ser293, Ser291) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-PDHA1/PDHA2 (Ser293, Ser291) polyclonal antibody (Product # PA5-106200) using a dilution of 1:200 (1 hr, 37°C), and followed by goat an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106200
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PDHA1(Accession P08559), corresponding to amino acid residues around phosphorylated Ser293.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of PDHA1/2 only when phosphorylated at Ser293/291.
Target Information
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PDHE1-A type I; PDHE1-A type II; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 alpha 2; PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A TYPE II); Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase E1alpha subunit
Gene Aliases: Pdha-1; Pdha-2; PDHA1; PDHA2; PDHAL; PHE1A
UniProt ID: (Mouse) P35486, (Mouse) P35487, (Rat) Q06437
Entrez Gene ID: (Rat) 29554, (Mouse) 18597, (Mouse) 18598, (Rat) 117098
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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