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Invitrogen
DACH1 Polyclonal Antibody
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FIGURE: 1 / 7
DACH1 Antibody (PA5-106272) in IHC (P)
Immunohistochemistry analysis of DACH1 in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with DACH1 polyclonal antibody (Product # PA5-106272) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-106272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human DACH1(Accession Q9UI36), corresponding to amino acid residues S160-L210.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total DACH1.
Target Information
Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dach1; dachshund family transcription factor 1; Dachshund homolog 1; FLJ10138; OTTHUMP00000040839; RP11-512J14.1
Gene Aliases: Dac; DACH; DACH1
UniProt ID: (Mouse) Q9QYB2
Entrez Gene ID: (Rat) 306096, (Mouse) 13134
transcription factor activity, RNA polymerase II core promoter sequence-specific binding involved in preinitiation complex assembly
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
DNA binding
protein binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
suckling behavior
regulation of transcription, DNA-templated
respiratory gaseous exchange
cell proliferation
negative regulation of transcription by competitive promoter binding
negative regulation of cell migration
development of primary female sexual characteristics
negative regulation of fibroblast proliferation
RNA polymerase II transcriptional preinitiation complex assembly
negative regulation of cell proliferation involved in contact inhibition
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
negative regulation of transcription, DNA-templated
negative regulation of DNA biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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