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          • Primary Antibodies ›
          • TRIM59 Antibodies

          Invitrogen

          TRIM59 Polyclonal Antibody

          Advanced Verification
          View all (3) TRIM59 antibodies

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          Cite TRIM59 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (2)
          • Advanced Verification (1)
          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          TRIM59 Antibody (PA5-106512) in ICC/IF

          Immunofluorescence analysis of Tripartite motif-containing protein 59 was performed using 70% confluent log phase THP-1 differentiated to M0 macrophages treated with 1 µg/mL LPS for 24h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRIM59 Polyclonal Antibody (Product # PA5-106512) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          TRIM59 Antibody in Immunocytochemistry (ICC/IF)
          TRIM59 Antibody in Western Blot (WB)
          TRIM59 Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          TRIM59 Polyclonal Antibody

          Product Details

          PA5-106512

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human TRIM59(Accession Q8IWR1), corresponding to amino acid residues K201-L251.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2854181

          Product Specific Information

          Antibody detects endogenous levels of total TRIM59.

          Target Information

          May serve as a multifunctional regulator for innate immune signaling pathways. [UniProt]

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: RING finger 1; RING finger protein 1; RING finger protein 104; Tripartite motif-containing protein 59; Tumor suppressor TSBF-1; tumor suppressor TSBF1 homolog

          View more View less

          Gene Aliases: 2310035M22Rik; 2700022F13Rik; Mrf1; RGD1311956; RNF104; TRIM57; TRIM59; TSBF1

          View more View less

          UniProt ID: (Mouse) Q922Y2

          View more View less

          Entrez Gene ID: (Mouse) 66949, (Rat) 365813

          View more View less

          Function(s)
          protein binding zinc ion binding metal ion binding ubiquitin protein ligase activity ubiquitin-protein transferase activity ubiquitin-protein ligase
          Process(es)
          protein ubiquitination negative regulation of I-kappaB kinase/NF-kappaB signaling innate immune response negative regulation of viral entry into host cell
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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