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Invitrogen
Phospho-LCK/FYN (Tyr416) Polyclonal Antibody
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Phospho-LCK/FYN (Tyr416) Antibody (PA5-110156) in WB
Western blot analysis of Phospho-LCK/FYN (Tyr416) in extracts of Jurkat cells. Samples were incubated with Phospho-LCK/FYN (Tyr416) Polyclonal antibody (Product # PA5-110156) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by UV at room temperature for 15-30 minutes. Lysates/proteins: 25 µg per lane. Blocking buf... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-110156
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.61 mg/mL
规格
61 µg
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: NEYTA
靶标信息
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found. Fyn is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. The unique N-terminal domain of Fyn interacts with the CD3 and Delta chains of the TcR. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; p56-LCK; p59-Fyn; Protein YT16; Proto-oncogene c-Fyn; Proto-oncogene Lck; Proto-oncogene Syn; SLK; Src-like kinase; T cell-specific protein-tyrosine kinase; Tyrosine-protein kinase Fyn; Tyrosine-protein kinase Lck
基因别名: FYN; LCK
Entrez Gene ID: (Human) 2534, (Human) 3932
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