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Invitrogen
PKA alpha Polyclonal Antibody
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图: 1 / 4
PKA alpha Antibody (PA5-115762) in ICC/IF
Immunocytochemistry analysis of PKA alpha in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115762) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115762
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PRKACA(Accession P17612), corresponding to amino acid residues L173-W223.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total PKA alpha CAT.
靶标信息
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C(s); C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit C alpha; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A; sperm cAMP-dependent protein kinase catalytic subunit
基因别名: Cs-PKA; PKACA; PKCA1; PKCD; PRKACA
UniProt ID: (Mouse) P05132
Entrez Gene ID: (Rat) 25636, (Mouse) 18747
protein kinase activity
protein serine/threonine kinase activity
cAMP-dependent protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
Rab GTPase binding
protein kinase binding
ubiquitin protein ligase binding
protein complex binding
protein kinase A regulatory subunit binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
mesoderm formation
neural tube closure
protein phosphorylation
positive regulation of cell proliferation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
regulation of cellular respiration
regulation of osteoblast differentiation
protein autophosphorylation
positive regulation of protein export from nucleus
sperm capacitation
modulation of synaptic transmission
negative regulation of meiotic cell cycle
regulation of synaptic transmission, glutamatergic
regulation of proteasomal protein catabolic process
regulation of protein processing
positive regulation of cell cycle arrest
cellular response to glucose stimulus
cellular response to parathyroid hormone stimulus
negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning
regulation of bicellular tight junction assembly
phosphorylation
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