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Invitrogen
SIGLEC9 Polyclonal Antibody
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SIGLEC9 Antibody (PA5-115900) in ICC/IF
Immunocytochemistry analysis of SIGLEC9 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115900) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-115900
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human SIGLEC9(Accession Q9Y336), corresponding to amino acid residues G383-Y433.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of total SIGLEC9.
靶标信息
SIGLEC9 (Sialic acid-binding Ig-like lectin 9) is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It contains two Ig-like C2 type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. It preferentially binds to alpha2, 3-or alpha2, 6-linked sialic acid. The sialic acid recognition site may be mased by cis interactions with sialic acids on the same cell surface.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD329; CDw329; Protein FOAP-9; Sialic acid-binding Ig-like lectin 9; Siglec; Siglec-9
基因别名: SIGLEC9; UNQ668/PRO1302
Entrez Gene ID: (Human) 27180
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