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Invitrogen
XPF Polyclonal Antibody
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XPF Antibody (PA5-117118) in WB
Western blot analysis of XPF in extracts of various cell lines. Samples were incubated with XPF Polyclonal antibody (Product # PA5-117118) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-117118
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.49 mg/mL
规格
149 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Positive Samples: U-251MG, HT-1080
Immunogen sequence: GYRREISSSP ESCPEEIKHE EFDVNLSSDA AFGILKEPLT IIHPLLGCSD PYALTRVLHE VEPRYVVLYD AELTFVRQLE IYRASRPGKP LRVYFLIYGG STEEQRYLTA LRKEKEAFEK LIREKASMVV PEEREGRDET NLDLVRGTAS ADVSTDTRKA GGQEQNGTQQ SIVVDMREFR SELPSLIHRR GIDIEPVTLE VGDYILTPEM CVERKSISDL IGSLNNGRLY SQCISMSRYY KRPVLLIEFD PSKPFSLTSR GALFQEISSN
靶标信息
The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DNA excision repair protein ERCC-4; DNA repair endonuclease subunit; DNA repair endonuclease XPF; DNA repair protein complementing XP-F cells; Ercc4 protein-like; excision repair cross-complementation group 4; excision repair cross-complementing rodent repair deficiency, complementation group 4; Xeroderma pigmentosum group F-complementing protein
基因别名: AI606920; ERCC11; ERCC4; RGD1560340; XPF
UniProt ID: (Mouse) Q9QZD4
Entrez Gene ID: (Mouse) 50505, (Rat) 304719
single-stranded DNA endodeoxyribonuclease activity
TFIID-class transcription factor binding
nucleic acid binding
DNA binding
damaged DNA binding
single-stranded DNA binding
nuclease activity
endonuclease activity
endodeoxyribonuclease activity
protein binding
protein C-terminus binding
hydrolase activity
phosphoric ester hydrolase activity
structure-specific DNA binding
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
protein N-terminus binding
DNA metabolism protein
endodeoxyribonuclease
nucleic acid metabolism protein
resolution of meiotic recombination intermediates
telomere maintenance
double-strand break repair via homologous recombination
DNA metabolic process
DNA repair
nucleotide-excision repair
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
cellular response to DNA damage stimulus
response to UV
UV protection
negative regulation of telomere maintenance
nucleotide-excision repair, DNA incision
cellular response to UV
nucleotide-excision repair involved in interstrand cross-link repair
DNA catabolic process, endonucleolytic
obsolete telomere maintenance via telomere shortening
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