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Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA Polyclonal Antibody
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Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA Antibody (PA5-117540) in WB
Western Blot analysis of sdeA using Legionella pneumophila subsp. pneumophila (strain Philadelphia 1/ATCC 33152/DSM 7513) sdeA Polyclonal Antibody (Product # PA5-117540) at 1:2,000. Positive WB detected in Recombinant protein. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-117540
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268aa)
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
2.88 mg/mL
规格
288 µg
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody has been tested in direct-ELISA
靶标信息
Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2). Transfers an ADP-ribose moiety from NAD to the 'Arg-42' residue of ubiquitin in a reaction that releases nicotinamide. The modified ubiquitin is subsequently transferred to the substrate protein through an unknown mechanism that results in the release of AMP. Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PubMed:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains ('Lys-11', 'Lys-48' and 'Lys-63') with a distinct preference for 'Lys-63' linkages; is thus able to efficiently remove 'Lys-63'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PubMed:26598703).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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