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Invitrogen
Phospho-RIP2 (Ser176) Polyclonal Antibody
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Phospho-RIP2 (Ser176) Antibody (PA5-118495) in ICC/IF
Immunocytochemical analysis of Phospho-RIP2 (Ser176) in heat-shocked Hela cells using a Phospho-RIP2 (Ser176) polyclonal antibody (Product # PA5-118495). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with the primary antibody and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated go... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-118495
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human RIPK2(Accession O43353), corresponding to amino acid residues around phosphorylated Ser176.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Antibody detects endogenous levels of RIPK2 only when phosphorylated at Sersine 176.
靶标信息
RIPK2 is a serine/threonine kinase that is involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; EC 2.7.11.1; growth-in; kinase RIPK2; receptor (TNFRSF)-interacting serine-threonine kinase 2; Receptor-interacting protein 2; Receptor-interacting serine/threonine-protein kinase 2; RIP 2; RIP-2; RIP-like; RIP-like-interacting CLARP kinase; Tyrosine-protein kinase RIPK2; WUGSC:H_RG437L15.1
基因别名: 2210420D18Rik; CARD3; CARDIAK; CCK; D4Bwg0615e; RICK; RIP2; RIPK2; UNQ277/PRO314/PRO34092
UniProt ID: (Mouse) P58801
Entrez Gene ID: (Rat) 362491, (Mouse) 192656
protein serine/threonine kinase activity
ATP binding
LIM domain binding
protein homodimerization activity
CARD domain binding
nucleotide binding
protein kinase activity
non-membrane spanning protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
positive regulation of cytokine-mediated signaling pathway
adaptive immune response
positive regulation of T-helper 1 type immune response
protein phosphorylation
I-kappaB kinase/NF-kappaB signaling
positive regulation of peptidyl-threonine phosphorylation
positive regulation of protein ubiquitination
lipopolysaccharide-mediated signaling pathway
positive regulation of chemokine production
positive regulation of interferon-gamma production
positive regulation of interleukin-2 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
positive regulation of stress-activated MAPK cascade
positive regulation of immature T cell proliferation
positive regulation of peptidyl-serine phosphorylation
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
T cell proliferation
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to exogenous dsRNA
innate immune response
positive regulation of T-helper 1 cell differentiation
positive regulation of JNK cascade
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-positive bacterium
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
positive regulation of ERK1 and ERK2 cascade
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
response to interleukin-1
response to interleukin-12
response to interleukin-18
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
cellular response to peptidoglycan
cellular response to muramyl dipeptide
immune system process
apoptotic process
phosphorylation
regulation of apoptotic process
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