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Invitrogen

Phospho-STAT4 (Tyr693) Polyclonal Antibody

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Phospho-STAT4 (Tyr693) Polyclonal Antibody

Product Details

PA5-121263

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around Y693 of human STAT4 (NP_003142.1).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Amount

72 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2914835

Product Specific Information

Positive test controls include: HeLa. The target is usually found in the following locations: Cytoplasm, Nucleus.

Immunogen sequence: KGYVP

Target Information

STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 is similar to other STAT family members in that it can be tyrosine phosphorylated by Jak1 or Jak2. STAT4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT4 can bind the IFN-gamma activated site (GAS). Serine phosphorylation of STAT is also required for maximal transcriptional activity. STAT4 expression is restricted to the thymus, spleen and testis. Until recently the cytokine(s) responsible for activation of STAT4 had not been identified. STAT4 is now known to be activated by the cytokine interleukin 12 (IL-12). IL-12 is required for the T-cell independent induction of IFN-gamma which is a key step in the initial suppression of bacterial and parasitic infections. In addition, IL-12 is required for the development of a Th1 response which is necessary for effective host defense against intracellular pathogens. STAT4-deficient mice display impaired IL-12 development of Th1 cells and enhanced development of Th2 cells. A recent study in mouse has shown that in response to viral infection IFN-a/b activation of STAT4 is required for IFN-g production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CIS4; HSPC060; OTTHUMP00000163553; SOCS4; SOCS6; SSI4; STAI4; STAT; STATI4; unnamed protein product

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Gene Aliases: DPMC; SLEB11

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UniProt ID: (Human) Q14765

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Entrez Gene ID: (Human) 6775, (Rat) 367264

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding identical protein binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding double-stranded DNA binding tumor necrosis factor receptor binding enzyme binding CCR5 chemokine receptor binding histone acetyltransferase binding histone binding protein homodimerization activity sequence-specific DNA binding ubiquitin-like protein ligase binding protein phosphatase 2A binding promoter-specific chromatin binding
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response immune response signal transduction JAK-STAT cascade cytokine-mediated signaling pathway interleukin-12-mediated signaling pathway regulation of cell proliferation response to peptide hormone T-helper 1 cell differentiation positive regulation of transcription from RNA polymerase II promoter response to interleukin-6 negative regulation of endothelial cell proliferation positive regulation of defense response to virus by host transcription, DNA-templated protein phosphorylation response to nutrient blood circulation biological_process cell proliferation positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus response to bacterium positive regulation of gene expression negative regulation of angiogenesis lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide positive regulation of interferon-alpha production cellular response to insulin stimulus tumor necrosis factor-mediated signaling pathway response to cytokine negative regulation of macrophage fusion response to type I interferon response to interferon-gamma response to interferon-beta cellular response to interferon-beta interleukin-7-mediated signaling pathway interleukin-9-mediated signaling pathway response to hydrogen peroxide regulation of apoptotic process positive regulation of apoptotic process negative regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated negative regulation by virus of viral protein levels in host cell positive regulation of smooth muscle cell proliferation negative regulation of developmental process response to cAMP defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway renal tubule development hematopoietic stem cell homeostasis interleukin-27-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interferon-gamma receptor signaling pathway via STAT
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