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Invitrogen

Phospho-RIP1 (Ser166) Polyclonal Antibody

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Phospho-RIP1 (Ser166) Polyclonal Antibody

Product Details

PA5-121351

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around S166 of human RIPK1/RIP (NP_003795.2).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.07 mg/mL

Amount

207 µg

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2914923

Product Specific Information

Positive test controls include: HT-29, NIH/3T3.

Immunogen sequence: MWSKL

Target Information

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell death protein RIP; FLJ39204; OTTHUMP00000015955; Receptor-interacting protein 1; Receptor-interacting serine/threonine-protein kinase 1; RIP-1; RIP1 (CT); serine/threonine-protein kinase RIP

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Gene Aliases: D330015H01Rik; Rinp; RIP; RIP1; RIPK1

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UniProt ID: (Mouse) Q60855

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Entrez Gene ID: (Mouse) 19766

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity death receptor binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding protein complex binding identical protein binding death domain binding non-receptor serine/threonine protein kinase
Process(es)
positive regulation of protein phosphorylation protein phosphorylation apoptotic process signal transduction positive regulation of cell death programmed cell death viral process phosphorylation positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production response to tumor necrosis factor peptidyl-serine autophosphorylation positive regulation of phosphorylation positive regulation of apoptotic process positive regulation of programmed cell death positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade cellular protein catabolic process positive regulation of macrophage differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade protein autophosphorylation positive regulation of NF-kappaB transcription factor activity protein homooligomerization protein heterooligomerization positive regulation of transferase activity positive regulation of necroptotic process T cell apoptotic process necroptotic process regulation of ATP:ADP antiporter activity cellular response to tumor necrosis factor cellular response to growth factor stimulus extrinsic apoptotic signaling pathway ripoptosome assembly necroptotic signaling pathway ripoptosome assembly involved in necroptotic process amyloid fibril formation regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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