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Invitrogen

Twist1/Twist2 Polyclonal Antibody

View all (2) Twist1/Twist2 antibodies

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Twist1/Twist2 Polyclonal Antibody

Product Details

PA5-143850

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence of human TWIST1/2 (KIIPTLPSDKLSKIQTLKLAARY).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075064

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: rat brain tissue, rat ovary tissue, mouse brain tissue, mouse ovary tissue. IHC: human penis squamous cell carcinoma tissue, human lung squamous cell carcinoma tissue, human lung cancer tissue, mouse brain tissue, rat brain tissue. Flow: THP-1 cell, RAW2647 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Twist1/Twist2 encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. Twist1/Twist2 is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: bHLHa38; bHLHa39; charlie chaplin; Class A basic helix-loop-helix protein 38; Class A basic helix-loop-helix protein 39; dermis expressed 1; Dermis-expressed protein 1; Dermo-1; dermo-1 protein; H-twist; M-twist; MGC117334; Pluridigite; polydactyly EMS; Skam10Jus; twist basic helix-loop-helix transcription factor 1; twist basic helix-loop-helix transcription factor 2; twist gene homolog 1; twist homolog 1; twist homolog 2; Twist-related protein 1; Twist-related protein 2

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Gene Aliases: AA960487; BHLHA38; BHLHA39; DERMO1; M-Twist; Pde; pdt; Ska10; Ska; TWIST; TWIST1; TWIST2

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UniProt ID: (Mouse) P26687, (Mouse) Q9D030, (Rat) P97831

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Entrez Gene ID: (Mouse) 22160, (Rat) 85489, (Mouse) 13345, (Rat) 59327

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity protein dimerization activity E-box binding molecular_function transcription factor activity, RNA polymerase II transcription factor binding chromatin binding basic helix-loop-helix transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoblast differentiation in utero embryonic development neuron migration neural tube closure aortic valve morphogenesis mitral valve morphogenesis endocardial cushion morphogenesis cardiac neural crest cell migration involved in outflow tract morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development muscle organ development positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of phosphatidylinositol 3-kinase signaling cell differentiation embryonic limb morphogenesis regulation of bone mineralization positive regulation of fatty acid beta-oxidation negative regulation of tumor necrosis factor production positive regulation of tumor necrosis factor production negative regulation of histone phosphorylation negative regulation of histone acetylation embryonic forelimb morphogenesis embryonic hindlimb morphogenesis hindlimb morphogenesis negative regulation of peroxisome proliferator activated receptor signaling pathway embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of molecular function negative regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of striated muscle tissue development negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process negative regulation of skeletal muscle tissue development embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis positive regulation of epithelial cell proliferation cranial suture morphogenesis embryonic camera-type eye formation eyelid development in camera-type eye cardiac neural crest cell development involved in outflow tract morphogenesis cellular response to hypoxia positive regulation of monocyte chemotactic protein-1 production positive regulation of DNA-templated transcription, initiation positive regulation of cell motility negative regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding negative regulation of cellular senescence positive regulation of interleukin-6 secretion negative regulation of double-strand break repair cell proliferation involved in heart valve development positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation odontogenesis palate development bone development cellular response to growth factor stimulus regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation positive regulation of keratinocyte proliferation negative regulation of DNA binding negative regulation of myeloid cell differentiation face morphogenesis cornea development in camera-type eye
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