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Invitrogen
Prostate Specific Acid Phosphatase Polyclonal Antibody
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FIGURE: 1 / 7
Prostate Specific Acid Phosphatase Antibody (PA5-143871) in ICC/IF
Immunocytochemistry analysis of Prostate Specific Acid Phosphatase in U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Prostate Specific Acid Phosphatase Polyclonal antibody (Product # PA5-143871) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-143871
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PAP/ACP3 recombinant protein (Position: E150-D386).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell. IHC: human prostate cancer tissue, mouse kidney tissue, rat kidney tissue. ICC/IF: U87 cell. Flow: HL-60 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ACPP is an enzyme which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-NT; 5'-nucleotidase; Acid phosphatase 3; EC 3.1.3.2; Ecto-5'-nucleotidase; Fluoride-resistant acid phosphatase; FRAP; lysosomal acid phosphatase; PAP; PPAP; prostate acid phosphatase; Prostatic acid phosphatase; prostatic acid phosphatase (rPAP); Protein tyrosine phosphatase ACP3; Thiamine monophosphatase; TMPase
Gene Aliases: 5'-NT; A030005E02Rik; ACP3; ACPP; Acpp11; FRAP; Lap; PAP; Ppal; RNACPP11
UniProt ID: (Mouse) Q8CE08, (Rat) P20646
Entrez Gene ID: (Mouse) 56318, (Rat) 56780
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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