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Invitrogen
UBE2D1/2/3/4 Polyclonal Antibody
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图: 1 / 12
UBE2D1/2/3/4 Antibody (PA5-143926) in ICC/IF
Immunocytochemistry analysis of UBE2D1/2/3/4 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in UBE2D1/2/3/4 Polyclonal antibody (Product # PA5-143926) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-143926
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.005mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat heart tissue, rat lung tissue, rat liver tissue, human HEK293 whole cell, human Jurkat whole cell, human CACO-2 whole cell. IHC: mouse lung lymph node tissue, human testicular cancer tissue, human bladder cancer tissue, rat lung tissue. ICC/IF: A431 cell. Flow: A431 cell, RAW2647 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: (E3-independent) E2 ubiquitin-conjugating enzyme D1; (E3-independent) E2 ubiquitin-conjugating enzyme D2; (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D2; E2 ubiquitin-conjugating enzyme D2B; E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D4; E2(17)KB 1; E2(17)KB 2; E2(17)KB 2B; E2(17)KB 3; HBUCE1; p53-regulated ubiquitin-conjugating enzyme 1; PAPase; Phosphoarginine phosphatase; SFT; Stimulator of Fe transport; ubc2e ubiquitin conjugating enzyme; UBC4/5 homolog; UbcH5; UBE2D1/UBE2D3/UBE2D3/UBE2D4; Ubiquitin carrier protein D1; Ubiquitin carrier protein D2; Ubiquitin carrier protein D2B; Ubiquitin carrier protein D3; Ubiquitin carrier protein D4; Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2 D2B; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2(17)KB 2B; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-conjugating enzyme E2-17 kDa 2B; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog; ubiquitin-conjugating enzyme E2D 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D1; Ubiquitin-protein ligase D2; Ubiquitin-protein ligase D2B; Ubiquitin-protein ligase D3; Ubiquitin-protein ligase D4
基因别名: 1100001F19Rik; 9430029A22Rik; AA414951; E217kB; PUBC1; RGD69425; SFT; Ubc2e; UBC4; UBC5A; UBC5B; UBC5C; UBCH4; UBCH5; UBCH5A; UBCH5B; UBCH5C; UBCH5D; UBE2D1; UBE2D2; Ube2d2b; UBE2D3; UBE2D4
UniProt ID: (Mouse) P61080, (Rat) D3ZDK2, (Rat) P62839, (Mouse) P61079, (Rat) P61078, (Rat) P70711
Entrez Gene ID: (Mouse) 216080, (Rat) 361831, (Rat) 641452, (Mouse) 66105, (Rat) 81920, (Rat) 79435
protein polyubiquitination
ubiquitin-dependent protein catabolic process
positive regulation of protein ubiquitination
protein K48-linked ubiquitination
metabolic process
protein ubiquitination
DNA repair
protein monoubiquitination
apoptotic process
cellular response to DNA damage stimulus
proteasome-mediated ubiquitin-dependent protein catabolic process
protein autoubiquitination
protein K11-linked ubiquitination
positive regulation of protein targeting to mitochondrion
biological_process
protein K29-linked ubiquitination
protein K27-linked ubiquitination
protein K63-linked ubiquitination
protein K6-linked ubiquitination
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