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Invitrogen
Mono-methyl-HIST1H2AG (Arg29) Polyclonal Antibody
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FIGURE: 1 / 1
Mono-methyl-HIST1H2AG (Arg29) Antibody (PA5-144309) in ICC/IF
Immunocytochemistry analysis of HeLa cells with Mono-methyl-HIST1H2AG (Arg29) polyclonal antibody (Product # PA5-144309) at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-144309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide sequence around site of Mono-methyl-Arg (29) derived from Human Histone H2A type 1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.15 mg/mL
Amount
15 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% Proclin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Histones are nuclear proteins that play an important role in maintaining the structure of the chromosome in eukaryotes. The nucleosome is made up of around 146bp of DNA, which enfolds around a histone octomer unit comprising of four major histones, H2A, H2B, H3 and H4. An important role of H2A is to ensure proper assembly or functioning of the centrometre. They also play an important role in gene regulation. The protein can be modified by acetylation and phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H2A.1; H2A/d; H2ak; HIST1H2AJ; Histone H2A type 1; histone H2A/e; Histone H2A/ptl
Gene Aliases: H2AC11; H2AC13; H2AC15; H2AC16; H2AC17; H2AFC; H2AFD; H2AFI; H2AFN; H2AFP; HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM
Entrez Gene ID: (Human) 8969, (Human) 8329
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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