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          • Primary Antibodies ›
          • NFkB p65 Antibodies

          Invitrogen

          Acetyl-NFkB p65 (Lys310) Polyclonal Antibody

          高级验证
          5个已发表图片
          7 篇参考文献
          查看其他94个NFkB p65抗体

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          Cite Acetyl-NFkB p65 (Lys310) Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (2)
          • 已发表图片 (5)
          • 高级验证 (1)
          Acetyl-NFkB p65 (Lys310) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Acetyl-NFkB p65 (Lys310) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 8

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          Acetyl-NFkB p65 (Lys310) Antibody (PA5-17264) in ICC/IF

          Immunofluorescence analysis of Acetyl-NFkB p65 (Lys310) was performed using 70% confluent log phase HCT 116 cells treated with SAHA (2 micromolar) for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-NFkB p65 (Lys310) Rabbit Polyclonal Antibo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          Acetyl-NFkB p65 (Lys310) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-NFkB p65 (Lys310) Antibody in Western Blot (WB)
          Acetyl-NFkB p65 (Lys310) Antibody in Immunohistochemistry (IHC)
          Acetyl-NFkB p65 (Lys310) Antibody in Immunocytochemistry (ICC/IF)
          Acetyl-NFkB p65 (Lys310) Antibody in Western Blot (WB)
          Acetyl-NFkB p65 (Lys310) Antibody in Western Blot (WB)
          Acetyl-NFkB p65 (Lys310) Antibody in Western Blot (WB)
          Acetyl-NFkB p65 (Lys310) Antibody

          产品信息

          PA5-17264

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:1,000
          查看4篇已发表文章 4篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:250
          查看1篇已发表文章 1篇已发表文章

          免疫沉淀 (IP)

          1:50
          -

          染色质免疫沉淀 实验 (ChIP)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Synthetic acetylated peptide corresponding to residues surrounding Lys310 of NFkB

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          62.1 µg/mL

          纯化类型

          Antigen affinity chromatography, Protein A

          保存液

          0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10983502

          产品详细信息

          It is not recommended to aliquot this antibody.

          靶标信息

          Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; EBP-1; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kB p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; unnamed protein product; v-rel avian reticuloendotheliosis viral oncogene homolog A

          查看更多 收起

          基因别名: AIF3BL3; CMCU; NFKB3; p65; RELA

          查看更多 收起

          UniProt ID: (Human) Q04206, (Mouse) Q04207

          查看更多 收起

          Entrez Gene ID: (Human) 5970, (Mouse) 19697

          查看更多 收起

          功能
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding protein binding enzyme binding protein kinase binding chromatin DNA binding ubiquitin protein ligase binding peptide binding phosphate ion binding identical protein binding protein homodimerization activity actinin binding histone deacetylase binding sequence-specific DNA binding macromolecular complex binding NF-kappaB binding ankyrin repeat binding general transcription initiation factor binding DNA-binding transcription factor binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor binding protein complex binding activating transcription factor binding transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding repressing transcription factor binding Rel homology transcription factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter negative regulation of cytokine production liver development hair follicle development defense response to tumor cell response to ischemia chromatin organization transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response inflammatory response cellular defense response neuropeptide signaling pathway I-kappaB kinase/NF-kappaB signaling positive regulation of cell proliferation response to xenobiotic stimulus response to mechanical stimulus response to bacterium organ morphogenesis response to UV-B positive regulation of vascular endothelial growth factor production positive regulation of gene expression positive regulation of Schwann cell differentiation negative regulation of angiogenesis cytokine-mediated signaling pathway signal transduction involved in regulation of gene expression protein catabolic process response to muramyl dipeptide response to lipopolysaccharide response to progesterone positive regulation of interleukin-1 beta production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production response to insulin tumor necrosis factor-mediated signaling pathway negative regulation of protein sumoylation response to cobalamin response to cytokine toll-like receptor 4 signaling pathway intracellular signal transduction cellular response to hepatocyte growth factor stimulus response to muscle stretch NIK/NF-kappaB signaling vascular endothelial growth factor signaling pathway toll-like receptor TLR6:TLR2 signaling pathway prolactin signaling pathway negative regulation of protein catabolic process response to hydrogen peroxide negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine innate immune response response to ethanol negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response positive regulation of T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to peptidoglycan cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor postsynapse to nucleus signaling pathway antiviral innate immune response negative regulation of cytokine production involved in inflammatory response negative regulation of NIK/NF-kappaB signaling positive regulation of NIK/NF-kappaB signaling positive regulation of beta-amyloid formation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter cellular response to angiotensin positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription from RNA polymerase II promoter response to organic substance positive regulation of chondrocyte differentiation positive regulation of interleukin-12 biosynthetic process cellular response to peptide hormone stimulus
          It has to be done as per old AB suggested Products section.

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