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Invitrogen
RhoGDI Polyclonal Antibody
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图: 1 / 5
RhoGDI Antibody (PA5-17475) in ICC/IF
Immunofluorescence analysis of RhoGDI was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RhoGDI Polyclonal Antibody (Product # PA5-17475) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-17475
产品规格
种属反应
Bovine,
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to human RhoGDI
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
39 µg/mL
规格
3.9 µg
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
靶标信息
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: arhgdia {ECO:0000312|RGD:1359547}; GDI-1; MGC117248; Rho GDI 1; rho GDI 1 {ECO:0000250|UniProtKB:P19803}; Rho GDI alpha; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP-dissociation inhibitor 1; rho GDP-dissociation inhibitor 1 {ECO:0000250|UniProtKB:P19803}; Rho-GDI alpha; rho-GDI alpha {ECO:0000250|UniProtKB:P19803}; unnamed protein product
基因别名: 5330430M07Rik; ARHGDIA; Bles01; C87222; Gdi-1; Gdi1; GDIA1; Rho-GDI; RhoDGI; RhoGDI-1
UniProt ID: (Bovine) P19803, (Rat) Q5XI73, (Mouse) Q99PT1
Entrez Gene ID: (Bovine) 338054, (Rat) 360678, (Mouse) 192662
Rho protein signal transduction
positive regulation of GTPase activity
semaphorin-plexin signaling pathway
regulation of cell shape
negative regulation of cell migration
regulation of protein localization
regulation of catalytic activity
cellular response to mechanical stimulus
cellular response to organic cyclic compound
cellular response to redox state
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