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Invitrogen
A-Raf Polyclonal Antibody
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A-Raf Antibody (PA5-17657)
IP-MS enrichment of A-RAF (LFQ intensity): A-RAF was enriched 229-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and A-RAF antibody (Product # PA5-17657). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of anti... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-17657
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues close to the linker domain of human A-Raf (aa 10-35).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
47 µg/mL
规格
4.7 µg
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with c-Raf or B-Raf.
靶标信息
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; raf-related oncogene; Ras-binding protein; RP1-230G1.1; Serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
基因别名: 1200013E08Rik; A-Raf; ARAF; ARAF1; AW495444; PKS; PKS2
UniProt ID: (Rat) P14056, (Mouse) P04627
Entrez Gene ID: (Rat) 64363, (Mouse) 11836
protein serine/threonine kinase activity
MAP kinase kinase kinase activity
receptor signaling protein activity
ATP binding
metal ion binding
nucleotide binding
protein kinase activity
protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
activation of MAPKK activity
protein phosphorylation
regulation of TOR signaling
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-serine phosphorylation
intracellular signal transduction
negative regulation of apoptotic process
signal transduction
phosphorylation
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