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Invitrogen
Phospho-C/EBP beta (Thr235) Polyclonal Antibody
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FIGURE: 1 / 2
Phospho-C/EBP beta (Thr235) Antibody (PA5-17686) in WB
Western blot was performed using anti-Phospho-C/EBP beta (Thr235) Polyclonal Antibody (Product # PA5-17686) and 36, 33 kDa bands corresponding to C/EBP beta (LAP isoforms) and 15 kDa band corresponding to C/EBP beta (LIP isoform) was observed to increase in 3T3-L1 upon LY294002 and Insulin treatment. Also, 15 kDa band corresponding to C/EBP beta (LIP isoform) was increased in Hep G2 upon LY294002 and Insuli... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17686
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding threonine 235 of human C/EBPβ
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
30 µg/mL
Amount
3 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other phosphorylated C/EBPs.
Target Information
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AGP/EBP; C EBP; C/EBP BETA; CCAAT/enhancer-binding protein beta; IL-6DBP; Interleukin-6-dependent-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; Liver-enriched transcriptional activator; Nuclear factor NF-IL6; nuclear protein Il6; OTTMUSP00000017503; TCF-5; Transcription factor 5
Gene Aliases: C/EBPbeta; CEBPB; CRP2; IL-6DBP; LAP; LIP; NF-IL6; NF-M; Nfil6; PP9092; TCF5
UniProt ID: (Mouse) P28033
Entrez Gene ID: (Mouse) 12608
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
transcription factor binding
kinase binding
histone acetyltransferase binding
glucocorticoid receptor binding
protein homodimerization activity
histone deacetylase binding
sequence-specific DNA binding
ubiquitin-like protein ligase binding
protein heterodimerization activity
basic leucine zipper transcription factor
ovarian follicle development
embryonic placenta development
granuloma formation
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell differentiation
neuron differentiation
response to lipopolysaccharide
positive regulation of interleukin-4 production
mammary gland epithelial cell proliferation
response to endoplasmic reticulum stress
T-helper 1 cell activation
negative regulation of T cell proliferation
defense response to bacterium
negative regulation of neuron apoptotic process
regulation of interleukin-6 biosynthetic process
fat cell differentiation
positive regulation of fat cell differentiation
positive regulation of osteoblast differentiation
regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
brown fat cell differentiation
mammary gland epithelial cell differentiation
regulation of transcription involved in cell fate commitment
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to amino acid stimulus
hepatocyte proliferation
liver regeneration
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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