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ERK2 Antibody (PA5-17710) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), C2C12 (Lane 2), HeLa (Lane 3), H1975 (Lane 4), A-431 (Lane 5), A549 (Lane 6), HCT 116 (Lane 7), HT-29 (Lane 8), MDA-MB-231 (Lane 9) and MCF7 (Lane 10). The blot was probed with Rabbit Anti-ERK2 Polyclonal Antibody (Product # PA5-17710, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band correspondin... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-17710
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to human p42 MAP kinase (Erk2)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
92 µg/mL
Amount
9.2 µg
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ERK-2; Erk2; cancer susceptibility protein; ERT1; extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAPK 1; MAPK 2; mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2; unnamed protein product
Gene Aliases: 9030612K14Rik; ERK; ERK-2; ERK2; ERT1; MAPK2; NS13; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2
UniProt ID: (Human) P28482, (Rat) P63086, (Mouse) P63085
Entrez Gene ID: (Human) 5594, (Rat) 116590, (Mouse) 26413
phosphotyrosine binding
protein kinase activity
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
kinase activity
phosphatase binding
identical protein binding
protein serine kinase activity
double-stranded DNA binding
protein kinase binding
mitogen-activated protein kinase kinase kinase binding
MAPK cascade
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
apoptotic process
chemotaxis
signal transduction
cell surface receptor signaling pathway
epidermal growth factor receptor signaling pathway
chemical synaptic transmission
learning or memory
insulin receptor signaling pathway
positive regulation of macrophage chemotaxis
positive regulation of peptidyl-threonine phosphorylation
Schwann cell development
positive regulation of telomere maintenance
regulation of stress-activated MAPK cascade
cellular response to amino acid starvation
response to nicotine
intracellular signal transduction
ERBB signaling pathway
ERBB2-ERBB3 signaling pathway
myelination
positive regulation of skeletal muscle tissue regeneration
positive regulation of cholesterol biosynthetic process
positive regulation of smoothened signaling pathway
insulin-like growth factor receptor signaling pathway
stress-activated MAPK cascade
regulation of cytoskeleton organization
interleukin-34-mediated signaling pathway
chemokine-mediated signaling pathway
ERK1 and ERK2 cascade
response to epidermal growth factor
caveolin-mediated endocytosis
regulation of Golgi inheritance
positive regulation of macrophage proliferation
positive regulation of neuroinflammatory response
regulation of early endosome to late endosome transport
cellular response to DNA damage stimulus
heart development
positive regulation of cell proliferation
response to toxic substance
organ morphogenesis
neural crest cell development
diadenosine tetraphosphate biosynthetic process
peptidyl-serine phosphorylation
sensory perception of pain
cytosine metabolic process
regulation of ossification
positive regulation of cell migration
thyroid gland development
lipopolysaccharide-mediated signaling pathway
response to estradiol
response to lipopolysaccharide
response to testosterone
mammary gland epithelial cell proliferation
cellular response to platelet-derived growth factor stimulus
response to cocaine
positive regulation of protein import into nucleus
outer ear morphogenesis
response to hydrogen peroxide
response to exogenous dsRNA
response to estrogen
estrous cycle
negative regulation of cell differentiation
positive regulation of translation
positive regulation of transcription, DNA-templated
decidualization
thymus development
T cell receptor signaling pathway
B cell receptor signaling pathway
regulation of intracellular pH
Bergmann glial cell differentiation
positive regulation of cardiac muscle cell proliferation
long-term synaptic potentiation
face development
lung morphogenesis
trachea formation
labyrinthine layer blood vessel development
cardiac neural crest cell development involved in heart development
cellular response to methionine
cellular response to cAMP
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to peptide hormone stimulus
cellular response to prostaglandin E stimulus
cellular response to lipid
cellular response to toxic substance
response to alcohol
cellular response to insulin-like growth factor stimulus
placenta development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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