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Invitrogen
IRAK4 Polyclonal Antibody
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图: 1 / 9
IRAK4 Antibody (PA5-20018) in ICC/IF
Immunofluorescence analysis of IRAK4 was performed using RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IRAK4 Polyclonal Antibody (Product # PA5-20018) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-20018
产品规格
种属反应
Human
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK-4.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
In Western blot applications, this antibody detects a band at ~50kDa. A suggested positive control is K562 cell lysate.
PA5-20018 can be used with blocking peptide PEP-0137.
靶标信息
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: interleukin-1
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
