Invitrogen

GOT1 Polyclonal Antibody

Name: GOT1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-24634
Availability: InStock

高级验证

2个已发表图片

3 篇参考文献

查看其他26个GOT1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

GOT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 6

GOT1 Antibody (PA5-24634) in IHC (P)

Immunohistochemistry analysis of GOT1 in formalin fixed and paraffin embedded brain tissue. Samples were incubated with GOT1 polyclonal antibody (Product # PA5-24634) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GOT1 Antibody — Glutaminolysis mediates miR-137-induced ferroptosis inhibition. a Schematic overview of the glutaminolysis pathway in ferroptosis. Glutamine (Gln) is transferred into cell cytoplasm by importers SLC1A5/SLC38A1, and subsequently converted to glutamate (Glu) by glutaminase GLS2 in mitochondria. The aspartate transaminase GOT1 ultimately converts Glu to a-ketoglutarate (a-KG), which contributes to ROS accumulation by an unknown mechanism. The small-molecule inhibitors are indicated in red: L-Gln transporter inhibitor GPNA; GLS inhibitor Compound 968; Pan-transaminases inhibitor AOA. b Pharmacological inhibition of multiple components in the glutaminolysis pathway abrogated anti-miR-137-mediated ferroptosis cell death and lipid accumulation. Cells transfected with control or miR-137 antagomirs were treated with erastin (5 µM) and GPNA (5 mM)/compound 968 (20 µM)/AOA (0.5 mM) for 24 h. The cell viability was assayed using a CCK-8 kit and the lipid accumulation was measured by MDA assay. Data shown represent mean ± SD from three independent experiments. n.s. nonsignificant; **p < 0.01. c Knockdown of Gln importers or metabolic enzymes blocked anti-miR-137-mediated ferroptosis cell death and lipid accumulation. Cells stably expressing control shRNA or indicated shRNA targeting metabolic enzymes were transfected with control or miR-137 antagomir, and then treated with erastin (5 µM) for 24 h. The cell viability was assayed using a CCK-8 kit and the lipid accumulation was measured by {KD}

Immunogen image

Immunogen Image GOT1 Polyclonal Antibody

产品信息

PA5-24634

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:10-1:50

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 352-381 amino acids from the C-terminal region of human GOT1

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

200 µg

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2542134

产品详细信息

This antibody is predicted to react with rat based on sequence homology.

靶标信息

GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: aspartate aminotransferase; aspartate aminotransferase 1; Aspartate aminotransferase, cytoplasmic; aspartate transaminase 1; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; Transaminase A; unnamed protein product

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基因别名: AST; AST1; ASTQTL1; cAspAT; cCAT; GIG18; GOT1; SGOT

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Entrez Gene ID: (Human) 2805

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功能

catalytic activity L-aspartate:2-oxoglutarate aminotransferase activity phosphatidylserine decarboxylase activity protein binding transaminase activity pyridoxal phosphate binding carboxylic acid binding L-cysteine:2-oxoglutarate aminotransferase activity (2S)-2-aminobutanoate:2-oxoglutarate transaminase activity

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