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Invitrogen
HMOX1 Polyclonal Antibody
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FIGURE: 1 / 19
HMOX1 Antibody (PA5-27338) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of HMOX1 was performed in Mock and treated HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HMOX1 Polyclonal Antibody (Product # PA5-27338) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27338
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human,
Mouse,
Rat,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Heme Oxygenase 1. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.07 mg/mL
Amount
107 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, Raw264.7, C2C12, rat spleen, HeLa (500 µM CoCl2 treatment for 24 hr).
Predicted reactivity: Mouse (81%), Rat (80%), Dog (85%), Pig (82%), Bovine (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein
Gene Aliases: D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HO; HO-1; HO1; Hsp32
UniProt ID: (Rat) P06762, (Mouse) P14901
Entrez Gene ID: (Rat) 24451, (Mouse) 15368
angiogenesis
response to hypoxia
wound healing involved in inflammatory response
phospholipid metabolic process
heme oxidation
response to oxidative stress
small GTPase mediated signal transduction
regulation of blood pressure
cell death
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of muscle cell apoptotic process
cellular response to nutrient
negative regulation of mast cell cytokine production
erythrocyte homeostasis
regulation of transcription from RNA polymerase II promoter in response to iron
cellular response to heat
response to nicotine
intracellular signal transduction
heme catabolic process
heme metabolic process
response to hydrogen peroxide
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of mast cell degranulation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of neuron apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
response to estrogen
positive regulation of angiogenesis
positive regulation of smooth muscle cell proliferation
negative regulation of smooth muscle cell proliferation
regulation of sequence-specific DNA binding transcription factor activity
protein homooligomerization
iron ion homeostasis
cellular response to arsenic-containing substance
cellular response to cadmium ion
cellular response to hypoxia
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
cellular iron ion homeostasis
apoptotic process
oxidation-reduction process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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