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Invitrogen
GAP43 Polyclonal Antibody
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FIGURE: 1 / 5
GAP43 Antibody (PA5-27448) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GAP43 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP43 Polyclonal Antibody (Product # PA5-27448) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27448
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human GAP43. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.55 mg/mL
Amount
55 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: IMR32, mouse hippocampus, rat hippocampus.
Predicted reactivity: Cat (83%), Chimpanzee (100%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GAP43 (Neuromodulin) is associated with nerve growth. GAP43 is expressed by developing and regenerating neurons, and to a lesser extent reactive glial cells. It is a major component of the motile "growth cones" that form the tips of elongating axons. GAP43 also plays a role in axonal and dendritic filopodia induction.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Axonal membrane protein GAP-43; brain abundant, membrane attached signal protein 2; Calmodulin-binding protein P-57; growth accentuating protein 43; Growth-associated protein 43; MGC156666; Neural phosphoprotein B-50; Neuromodulin; neuron protein F1; pp46; Protein F1
Gene Aliases: B-50; Basp2; GAP-43; GAP43
UniProt ID: (Rat) P07936, (Mouse) P06837
Entrez Gene ID: (Rat) 29423, (Mouse) 14432
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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