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Invitrogen
Ku70 Polyclonal Antibody
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FIGURE: 1 / 11
Ku70 Antibody (PA5-27538) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Ku70 in HeLa cells fixed with 4% paraformaldehyde at RT for 15 min. Green: Ku70 Polyclonal Antibody (Product # PA5-27538) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27538
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2.
Predicted reactivity: Mouse (82%), Rat (83%), Pig (87%), Chimpanzee (100%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
XRCC6 is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 5'-dRP/AP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CT; CTA-216E10.7; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein Ku70; DNA repair protein XRCC6; Ku autoantigen protein p70 homolog; Ku p70; Ku70; Lupus Ku autoantigen protein p70; OTTHUMP00000028581; thyroid autoantigen 70 kDa; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6
Gene Aliases: 70kDa; G22P1; Ku70; XRCC6
UniProt ID: (Mouse) P23475
Entrez Gene ID: (Mouse) 14375
nucleotide binding
DNA binding
damaged DNA binding
double-stranded DNA binding
double-stranded telomeric DNA binding
catalytic activity
ATP-dependent DNA helicase activity
helicase activity
ATP binding
protein C-terminus binding
hydrolase activity
lyase activity
telomeric DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
5'-deoxyribose-5-phosphate lyase activity
DNA helicase
telomere maintenance
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
DNA recombination
transcription, DNA-templated
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
metabolic process
response to ionizing radiation
V(D)J recombination
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of neurogenesis
cellular hyperosmotic salinity response
cellular response to X-ray
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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