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Invitrogen
TOP2B Polyclonal Antibody
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FIGURE: 1 / 8
TOP2B Antibody (PA5-27750) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TOP2B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-27750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.48 mg/mL
Amount
48 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Jurkat, K562, THP-1.
Predicted reactivity: Mouse (89%), Rat (86%), Pig (88%), Rhesus Monkey (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA topoisomerase 2-beta; DNA topoisomerase II, beta isozyme; topoisomerase II beta
Gene Aliases: D230016L12Rik; Top-2; TOP2B
UniProt ID: (Mouse) Q64511
Entrez Gene ID: (Rat) 361100, (Mouse) 21974
chromatin binding
DNA topoisomerase type II (ATP-hydrolyzing) activity
protein kinase C binding
protein C-terminus binding
enzyme binding
histone deacetylase binding
protein heterodimerization activity
nucleotide binding
DNA binding
DNA topoisomerase activity
protein binding
ATP binding
DNA binding, bending
isomerase activity
metal ion binding
DNA metabolism protein
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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