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Invitrogen
HSP70 Polyclonal Antibody
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图: 1 / 10
HSP70 Antibody (PA5-28369) in ICC/IF
Immunofluorescence analysis of HSPA1B was performed using 70% confluent log phase PC-3 cells treated with Heat Shock (43 degree celsius for 1 hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HSPA1B Polyclonal Antibody (Product # PA5-28369) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ S... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-28369
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human Hsp70. The exact sequence is proprietary.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.55 mg/mL
规格
55 µg
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 10% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS.
Predicted reactivity: Mouse (96%), Rat (98%), Xenopus laevis (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
HSP70 is a Heat shock protein (HSP) which are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs that include HSP70, there are HSP90 and HSP100. The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP70 family members include: HSP70, a protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein that is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa, constitutively expressed, protein which is involved in the uncoating of clathrin coated vesicles; GRP78, or BiP, is a glucose regulated 78 kDa protein localized in the endoplasmic reticulum; and p75, or HSP75, a 75 kDa protein that is found within the mitochondria. Further, HSP70 is encoded by an intronless gene and, in conjunction with other heat shock proteins, HSP70 stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and organelles. HSP70 is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The HSP70 gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 70 kilodalton heat shock protein; ARABIDOPSIS HEAT SHOCK PROTEIN 70; ATHSP70; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 1-Hom; Heat shock 70 kDa protein 1-like; heat shock 70 kDa protein 1/2; Heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 1L; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; Heat shock 70 kDa protein 4; Heat shock 70-related protein APG-2; heat shock 70kD protein 1B; heat shock 70kDa protein 2; Heat shock protein; heat shock protein 70; heat shock protein 70-1; Heat shock protein 70-1l; Heat shock protein 70.2; heat shock protein alpha 2; Heat shock protein family A member 1A; Heat shock protein family A member 1B; Heat shock protein family A member 1L; Heat shock protein family A member 2; Heat shock protein family H member 2; Heat shock-related 70 kDa protein 2; HEL S 103; HSP; HSP70 1; HSP70 1B; HSP70 2; HSP70 AceyltK77; HSP70-1; HSP70-1/HSP70-2; HSP70-2; HSP70-Hom; HSP70.1/2; HSP70.1/HSP70.2; HSP70.3; HSP70RY; HSPA2; HSPA4; Hspa70; HST; Ischemia responsive 94 kDa protein; Testis-specific heat shock protein-related; testis-specific heat shock protein-related gene hst70
基因别名: APG2; Hsp110; Hsp70; Hsp70-1; Hsp70-2; Hsp70-3; HSP72; HSPA1; HSPA1A; HSPA1B; HSPA1L; HSPA2; HSPA4; HSPH2; Hspt70; Hst70; HSX70; Irp94
UniProt ID: (Rat) P0DMW0, (Rat) P55063, (Rat) P14659, (Rat) O88600
Entrez Gene ID: (Rat) 24472, (Rat) 294254, (Rat) 24963, (Rat) 60460, (Rat) 266759
G-protein coupled receptor binding
protease binding
double-stranded RNA binding
protein binding
ATP binding
ATPase activity
enzyme binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
protein binding involved in protein folding
poly(A) RNA binding
protein N-terminus binding
NF-kappaB binding
unfolded protein binding
molecular_function
glycolipid binding
RNA polymerase II transcription factor binding
protein complex binding
activating transcription factor binding
positive regulation of protein phosphorylation
male meiosis
male meiosis I
multicellular organism development
spermatid development
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle
protein refolding
synaptonemal complex disassembly
negative regulation of inclusion body assembly
binding of sperm to zona pellucida
biological_process
positive regulation of T cell mediated cytotoxicity
mRNA catabolic process
defense response
response to unfolded protein
negative regulation of cell proliferation
response to heat
regulation of cell death
negative regulation of cell growth
negative regulation of protein ubiquitination
negative regulation of myeloid cell apoptotic process
cellular response to heat
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of erythrocyte differentiation
negative regulation of vasoconstriction
negative regulation of cell death
cellular heat acclimation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
telomere maintenance
response to ischemia
DNA repair
response to radiation
response to mechanical stimulus
neuron differentiation
response to ethanol
positive regulation of neuron differentiation
kidney development
negative regulation of protein phosphorylation
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of DNA binding
protein import into mitochondrial outer membrane
positive regulation of angiogenesis
chaperone-mediated protein complex assembly
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