Invitrogen

PGK1 Polyclonal Antibody

Name: PGK1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-28612
Availability: InStock

高级验证

21个已发表图片

23 篇参考文献

查看其他21个PGK1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

PGK1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 33

PGK1 Antibody (PA5-28612) in ICC/IF

Immunofluorescence analysis of PGK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PGK1 Polyclonal Antibody (Product # PA5-28612) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a d... View More

PGK1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

PGK1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PGK1 siRNA and loss of signal was observed in Western Blot using Anti-PGK 1 Polyclonal Antibody (Product # PA5-28612). {KD}

产品信息

PA5-28612

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看22篇已发表文章 22篇已发表文章

免疫细胞化学 (ICC/IF)

1:20-1:1,000

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

Proximity Ligation Assay (PLA) (PLA)

Assay-dependent

产品规格

种属反应

Human, Mouse, Non-human primate, Rat, Yeast

已发表种属

Human, Yeast

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.12 mg/mL

12 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546088

产品详细信息

Recommended positive controls: 293T, PGK1 shRNA-transfected 293T, A431, HeLa, HepG2, mouse brain, Rat liver, Yeast wild type 303.

Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (87%), Xenopus laevis (84%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Also known as ATP:3-phosphoglycerate 1-phosphotransferase showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。