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Invitrogen
TIGAR Polyclonal Antibody
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FIGURE: 1 / 5
TIGAR Antibody (PA5-29151) in ICC/IF
Immunofluorescence analysis of TIGAR was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIGAR Polyclonal Antibody (Product # PA5-29151) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More
Product Details
PA5-29151
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TIGAR. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the kelch family of proteins, which are characterized by kelch repeat motifs and a POZ/BTB protein-binding domain. It is thought that kelch repeats are actin binding domains. However, the specific function of this protein has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; transactivated by NS3TP2 protein
Gene Aliases: C12orf5; FR2BP; TIGAR
UniProt ID: (Human) Q9NQ88
Entrez Gene ID: (Human) 57103
response to ischemia
fructose 2,6-bisphosphate metabolic process
autophagy
apoptotic process
cellular response to DNA damage stimulus
response to xenobiotic stimulus
response to gamma radiation
positive regulation of cardiac muscle cell apoptotic process
dephosphorylation
fructose 1,6-bisphosphate metabolic process
negative regulation of programmed cell death
regulation of pentose-phosphate shunt
positive regulation of DNA repair
negative regulation of glycolytic process
intestinal epithelial cell development
cellular response to cobalt ion
cellular response to hypoxia
negative regulation of neuron death
negative regulation of macromitophagy
regulation of response to DNA damage checkpoint signaling
positive regulation of hexokinase activity
negative regulation of glucose catabolic process to lactate via pyruvate
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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