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Invitrogen
HAT1 Polyclonal Antibody
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FIGURE: 1 / 4
HAT1 Antibody (PA5-29174) in ICC/IF
HAT1 Polyclonal Antibody [N1C3-2] detects HAT1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAT1 protein stained by HAT1 Polyclonal Antibody [N1C3-2] (Product # PA5-29174) diluted at 1:500. Blue: Hoechst 33342 staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29174
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human HAT1 Recombinant protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.98 mg/mL
Amount
98 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: U87-MG.
Predicted reactivity: Mouse (92%), Rat (95%), Chicken (83%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; Histone methacryltransferase HAT1
Gene Aliases: HAT1; KAT1
Entrez Gene ID: (Human) 8520
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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