Invitrogen

Ezrin Polyclonal Antibody

Name: Ezrin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-29358
Availability: InStock

高级验证

1 篇参考文献

查看其他37个Ezrin抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (9)
高级验证 (2)

Ezrin Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

Ezrin Antibody (PA5-29358) in ICC/IF

Ezrin Polyclonal Antibody detects Ezrin protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: Ezrin protein stained by Ezrin Polyclonal Antibody (Product # PA5-29358) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Ezrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Ezrin Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in HeLa Cas9 cell line transduced with Ezrin Lentiviral sgRNA compared to control cell line using Anti-Ezrin Polyclonal Antibody (Product # PA5-29358). {KO}

产品信息

PA5-29358

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:20,000

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat, Zebrafish

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.84 mg/mL

184 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2546834

产品详细信息

Recommended positive controls: 293T, A431, HeLa.

Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (85%), Dog (98%), Rabbit (94%), Chicken (92%), Chimpanzee (99%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。