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Invitrogen
ALDH2 Polyclonal Antibody
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FIGURE: 1 / 7
ALDH2 Antibody (PA5-29717) in ICC/IF
Immunofluorescence analysis of ALDH2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH2 Polyclonal Antibody (Product # PA5-29717) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-29717
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat,
Zebrafish
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A431, HeLa, HepG2, mouse brain.
Predicted reactivity: Mouse (97%), Rat (96%), Xenopus laevis (83%), Pig (92%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ALDH2 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Asians have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Asians than among Caucasians could be related to the absence of the mitochondrial isozyme.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 (EC 1.2.1.3); aldehyde dehydrogenase 2 family (mitochondrial); aldehyde dehydrogenase 2, mitochondrial; aldehyde dehydrogenase precursor (ALDH; EC 1.2.1.3); ALDH class 2; ALDH-E2; ALDH1; ALDH2; EC 1.2.1.3; epididymis secretory sperm binding protein; liver mitochondrial ALDH; MGC1806; mitochondrial aldehyde dehydrogenase; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; unnamed protein product
Gene Aliases: Ahd-5; AHD-M1; Ahd5; ALDH-E2; ALDHI; ALDM
UniProt ID: (Human) P05091, (Mouse) P47738, (Rat) P11884
Entrez Gene ID: (Human) 217, (Mouse) 11669, (Rat) 29539
retinal dehydrogenase activity
aldehyde dehydrogenase (NAD) activity
phenylacetaldehyde dehydrogenase (NAD+) activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
NAD binding
carboxylesterase activity
acetaldehyde dehydrogenase (NAD+) activity
protein binding
identical protein binding
NADH binding
ethanol metabolic process
ethanol catabolic process
nitroglycerin metabolic process
dopamine catabolic process
aldehyde catabolic process
cellular detoxification of aldehyde
acetaldehyde metabolic process
intrinsic apoptotic signaling pathway in response to oxidative stress
negative regulation of apoptotic process
behavioral response to ethanol
regulation of reactive oxygen species metabolic process
liver development
response to ischemia
apoptotic mitochondrial changes
response to estradiol
response to lipopolysaccharide
response to progesterone
cellular response to hormone stimulus
response to testosterone
response to nicotine
response to ethanol
gland development
response to hyperoxia
cellular response to fatty acid
regulation of response to oxidative stress
regulation of dopamine biosynthetic process
cellular response to resveratrol
regulation of serotonin biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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