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Invitrogen
Calpain 3 Polyclonal Antibody
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Product Details
PA5-29721
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 3. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.43 mg/mL
Amount
43 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: H1299, HeLa, HepG2, Molt-4.
Predicted reactivity: Mouse (86%), Rat (89%), Pig (88%), Sheep (87%), Bovine (88%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calpain, an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins, plays a role in cytoskeletal reorganization and muscle protein degradation. Calpain is a heterodimer composed of a small regulatory subunit and one of three large catalytic subunits, designated calpain 1, calpain 2 and calpain 3. Calpain 3 (calpain p94) is a muscle-preferred calcium activated neutral protease that localizes to the nucleus. Mutations involving the calpain 3 gene are associated with limb-girdle muscle dystrophy type 2A, a form of autosomal recessive and progressive neuromuscular diso4. Calpastatin regulates calpain by inhibiting both the proteolytic activity of calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcium dependent cysteine protease; Calcium-activated neutral proteinase 3; calpain 3 (p94) isoform CAPN3_1; calpain 3 (p94) isoform CAPN3_2; calpain 3 (p94) isoform CAPN3_7; Calpain L3; Calpain p94; calpain p94, large [catalytic] subunit; calpain, large polypeptide L3; Calpain-3; CANP 3; CAPN 3; cysteine protease; hMp78; cysteine protease; hMp84; LGMD 2A; MGC10767; MGC11121; MGC14344; MGC4403; Muscle-specific calcium-activated neutral protease 3; muscle-specific calcium-activated neutral protease 3 large subunit; nCL-1; New calpain 1; unnamed protein product
Gene Aliases: CANP3; CANPL3; CAPN3; LGMD2; LGMD2A; LGMDD4; LGMDR1; nCL-1; NCL1; p94
UniProt ID: (Human) P20807
Entrez Gene ID: (Human) 825
catalytic activity
calcium-dependent cysteine-type endopeptidase activity
calcium ion binding
protein binding
peptidase activity
cysteine-type peptidase activity
structural constituent of muscle
hydrolase activity
sodium ion binding
titin binding
identical protein binding
metal ion binding
ligase regulator activity
binding, bridging
proteolysis
apoptotic process
muscle organ development
programmed cell death
positive regulation of satellite cell activation involved in skeletal muscle regeneration
response to muscle activity
protein catabolic process
myofibril assembly
protein destabilization
negative regulation of protein sumoylation
negative regulation of apoptotic process
regulation of I-kappaB kinase/NF-kappaB signaling
sarcomere organization
regulation of myoblast differentiation
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
muscle cell cellular homeostasis
positive regulation of release of sequestered calcium ion into cytosol
response to calcium ion
muscle structure development
protein-containing complex assembly
G1 to G0 transition involved in cell differentiation
cellular response to calcium ion
cellular response to salt stress
protein localization to membrane
self proteolysis
calcium-dependent self proteolysis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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