Invitrogen

SMYD3 Polyclonal Antibody

Name: SMYD3 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-31919
Availability: InStock

高级验证

4个已发表图片

6 篇参考文献

查看其他15个SMYD3抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

SMYD3 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

SMYD3 Antibody (PA5-31919) in ICC/IF

SMYD3 Polyclonal Antibody detects SMYD3 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 Polyclonal Antibody (Product # PA5-31919) diluted at 1:500. Blue: Hoechst 33342 staining.

SMYD3 Antibody in Immunoprecipitation (IP)

SMYD3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

SMYD3 Antibody — Fig 5 Gene ablation techniques show no dependence on SMYD2 or SMYD3 for cancer cell proliferation. Waterfall plot representing LogP RSA scores for sgRNAs targeting A) SMYD2 and B) SMYD3. 313 cell lines were infected with a library of 6500 sgRNAs targeting 600 different genes. LogP RSA scores represent depletion of guides from an infected cell population. Each bar represents a different cell line. Bars are colored by cancer subtype. C) Percent confluency of Hep3B cells infected with CRISPR viruses containing CAS9 and sgRNAs targeting HBE-1, EZH2 (negative controls) or SMYD3. Cell density was evaluated using an Incucyte Zoom. Growth curves were initiated 24 days following virus infection and puromycin selection. Plotted data is the average of three biological replicates. Error bars represent standard deviation (not readily visible on scale). D) SMYD3 western blot of lysates derived from Hep3B cells infected with CAS9 and SMYD3 sgRNA. Parental Hep3Bs and Hep3Bs stably infected with HBE-1, EZH2 (negative controls) or SMYD3 were lysed and probed for SMYD3 levels by western. GAPDH levels were evaluated as a loading control. {KO}

产品信息

PA5-31919

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.23 mg/mL

123 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2549392

产品详细信息

Recommended positive controls: DDDDK-tagged SMYD3-transfected 293T, 293T, A431, HeLa, HepG2, A549, SKOV3, HCT116.

Predicted reactivity: Human (99%), Mouse (93%), Rat (94%), Pig (95%), Rhesus Monkey (99%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger, MYND domain containing 1

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基因别名: 2410008A19Rik; SMYD3; ZMYND1; ZNFN3A1

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UniProt ID: (Mouse) Q9CWR2

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Entrez Gene ID: (Mouse) 69726

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功能

RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II core binding RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding protein binding methyltransferase activity transferase activity histone-lysine N-methyltransferase activity metal ion binding histone modifying enzyme

参与通路