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          • Primary Antibodies ›
          • Connexin 43 Antibodies

          Invitrogen

          Connexin 43 Polyclonal Antibody

          View all (33) Connexin 43 antibodies

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          Cite Connexin 43 Polyclonal Antibody

          Additional Information:

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          • Antibody Testing Data (1)
          Connexin 43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          Connexin 43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Connexin 43 Antibody (PA5-32778) in IHC (P)

          Immunohistochemistry analysis of Connexin 43 in human testis, seminiferous tubules and Leydig cells. Samples were incubated with Connexin 43 polyclonal antibody (Product # PA5-32778). Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Connexin 43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Connexin 43 Polyclonal Antibody

          Product Details

          PA5-32778

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          10-20 µg/mL
          -
          Product Specifications

          Species Reactivity

          Bovine, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic 16 amino acid peptide from C-terminus of human GJA1 / Connexin 43.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          50 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS

          Contains

          0.1% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2550240

          Product Specific Information

          Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Rabbit, Pig, Opossum, Guinea pig (100%); Bat, Horse, Chicken, Lizard, Xenopus (94%); Trout, Salmon (88%); Zebrafish, Sea anemone, Nematode (81%).

          For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

          Target Information

          Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: alpha 1 connexin; connexin 43; connexin k1; Connexin-43; Cx-43; cx43; CXA1; DFNB38; gap junction 43 kDa heart protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein connexin 43; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); GJA1; Npm1; ODD; ODOD; SDTY3; Syndactyly type III; unnamed protein product; vascular smooth muscle connexin-43; vascular smooth muscle connexin43; VEGF

          View more View less

          Gene Aliases: AVSD3; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxnk1; EKVP; EKVP3; Gja-1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

          View more View less

          UniProt ID: (Rabbit) Q6TYA7, (Human) P17302, (Bovine) P18246, (Mouse) P23242, (Rat) P08050

          View more View less

          Entrez Gene ID: (Rabbit) 100008935, (Pig) 100518636, (Human) 2697, (Dog) 403418, (Bovine) 281193, (Mouse) 14609, (Rat) 24392, (Chinese hamster) 100689077, (Guinea pig) 100379273

          View more View less

          Function(s)
          tubulin binding gap junction hemi-channel activity receptor binding gap junction channel activity protein binding beta-catenin binding ion transmembrane transporter activity efflux transmembrane transporter activity transmembrane transporter activity glutathione transmembrane transporter activity alpha-tubulin binding beta-tubulin binding gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling protein tyrosine kinase binding SH3 domain binding PDZ domain binding channel activity protein domain specific binding connexin binding disordered domain specific binding gap junction
          Process(es)
          response to ischemia spermatogenesis heart development response to wounding negative regulation of cell growth cellular response to platelet-derived growth factor stimulus regulation of apoptotic process bone remodeling transmembrane transport bone development positive regulation of smooth muscle cell chemotaxis microtubule-based transport establishment of mitotic spindle orientation cardiac conduction system development cell communication signal transduction cell-cell signaling protein localization male gonad development positive regulation of gene expression cell communication by electrical coupling glutamate secretion gap junction assembly negative regulation of gonadotropin secretion ion transmembrane transport glutathione transmembrane transport maintenance of blood-brain barrier T cell proliferation T cell activation xenobiotic transport positive regulation of I-kappaB kinase/NF-kappaB signaling cell-cell junction organization cellular response to pH atrial cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction positive regulation of cold-induced thermogenesis export across plasma membrane negative regulation of trophoblast cell migration regulation of blood circulation cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of morphogenesis of an epithelium positive regulation of mesodermal cell differentiation positive regulation of stem cell proliferation in utero embryonic development neuron migration heart looping epithelial cell maturation adult heart development regulation of heart contraction negative regulation of cell proliferation response to pH ATP transport cellular protein localization embryonic heart tube development skeletal muscle tissue regeneration positive regulation of protein catabolic process positive regulation of striated muscle tissue development blood vessel morphogenesis neuron projection morphogenesis protein oligomerization osteoblast differentiation negative regulation of endothelial cell proliferation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration atrial ventricular junction remodeling epicardial cell to mesenchymal cell transition positive regulation of cytosolic calcium ion concentration vascular transport negative regulation of gene expression cell communication by chemical coupling regulation of cell communication by electrical coupling positive regulation of cell communication by chemical coupling regulation of bone mineralization positive regulation of insulin secretion maintenance of protein localization in endoplasmic reticulum vasodilation embryonic digit morphogenesis positive regulation of meiotic nuclear division positive regulation of vasoconstriction regulation of bone remodeling regulation of body fluid levels regulation of calcium ion transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of blood vessel remodeling regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization cardiac conduction establishment of epithelial cell polarity regulation of actin filament organization positive regulation of establishment of Sertoli cell barrier negative regulation of DNA biosynthetic process regulation of bicellular tight junction assembly positive regulation of behavioral fear response endothelium development response to glucose response to estradiol response to lipopolysaccharide response to retinoic acid response to fluid shear stress response to peptide hormone decidualization cellular response to mechanical stimulus cellular response to parathyroid hormone stimulus epididymis development
          It has to be done as per old AB suggested Products section.

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