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Invitrogen
Pellino 1 Polyclonal Antibody
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Product Details
PA5-34465
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 15 amino acid peptide near the carboxy terminus of human Pellino 1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is human liver tissue lysate.
PA5-34465 can be used with blocking peptide PEP-1507.
Target Information
The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-Kappa-B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-epsilon; and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase pellino homolog 1; pellino 1; pellino homolog 1; Pellino-1; Pellino-related intracellular-signaling molecule; pellino1; protein pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1; unnamed protein product
Gene Aliases: 2810468L03Rik; A930031K15Rik; AA409794; AI586297; D11Ertd676e; PELI1; PRISM
UniProt ID: (Human) Q96FA3, (Mouse) Q8C669
Entrez Gene ID: (Human) 57162, (Mouse) 67245, (Rat) 305549
ubiquitin-protein transferase activity
protein binding
transferase activity
ubiquitin-ubiquitin ligase activity
ubiquitin protein ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
double-strand break repair via homologous recombination
positive regulation of cytokine production
DNA repair
cellular response to DNA damage stimulus
regulation of Toll signaling pathway
protein ubiquitination
positive regulation of B cell proliferation
positive regulation of protein ubiquitination
response to lipopolysaccharide
positive regulation of toll-like receptor 3 signaling pathway
positive regulation of toll-like receptor 4 signaling pathway
TORC1 signaling
T cell proliferation
T cell activation
negative regulation of T cell proliferation
positive regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
response to dsRNA
negative regulation of T cell activation
positive regulation of B cell activation
regulation of necroptotic process
negative regulation of necroptotic process
protein K63-linked ubiquitination
protein K48-linked ubiquitination
negative regulation of TORC1 signaling
positive regulation of TORC1 signaling
positive regulation of double-strand break repair via homologous recombination
Toll signaling pathway
negative regulation of NF-kappaB transcription factor activity
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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