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Invitrogen
TRAP220 Polyclonal Antibody
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TRAP220 Antibody (PA5-36114) in ICC/IF
Immunofluorescence analysis of TRAP220 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRAP220 Polyclonal Antibody (Product # PA5-36114) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-36114
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to amino acids 1420-1470 of human TRAP220
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody detects endogenous protein at a molecular weight of 220 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; PBP; peroxisome proliferator activated receptor binding protein; Peroxisome proliferator-activated receptor-binding protein; Ppar binding protein; PPAR-binding protein; PPRB; Thyroid hormone receptor-associated protein complex 220 kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2
基因别名: AI480703; CRSP210; DRIP205; l11Jus15; Med1; PBP; Pparbp; TRAP220; Trip2
UniProt ID: (Mouse) Q925J9
Entrez Gene ID: (Mouse) 19014
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
RNA polymerase II transcription cofactor activity
DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
receptor activity
protein binding
transcription factor binding
ligand-dependent nuclear receptor binding
estrogen receptor binding
ligand-dependent nuclear receptor transcription coactivator activity
thyroid hormone receptor coactivator activity
chromatin DNA binding
protein complex binding
nuclear hormone receptor binding
mediator complex binding
vitamin D receptor binding
retinoic acid receptor binding
peroxisome proliferator activated receptor binding
thyroid hormone receptor binding
LBD domain binding
ubiquitin protein ligase activity
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
angiogenesis
in utero embryonic development
liver development
embryonic placenta development
lens development in camera-type eye
thyroid hormone mediated signaling pathway
ventricular trabecula myocardium morphogenesis
retinal pigment epithelium development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase I promoter
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
thyroid hormone generation
androgen biosynthetic process
multicellular organism development
brain development
heart development
lactation
positive regulation of cell proliferation
organ morphogenesis
positive regulation of gene expression
negative regulation of keratinocyte proliferation
protein ubiquitination
keratinocyte differentiation
monocyte differentiation
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
organ regeneration
positive regulation of intracellular estrogen receptor signaling pathway
positive regulation of protein import into nucleus, translocation
mammary gland epithelial cell proliferation
positive regulation of mammary gland epithelial cell proliferation
embryonic heart tube development
embryonic hindlimb morphogenesis
embryonic hemopoiesis
peroxisome proliferator activated receptor signaling pathway
cellular response to hepatocyte growth factor stimulus
megakaryocyte development
mRNA transcription from RNA polymerase II promoter
camera-type eye development
negative regulation of apoptotic process
fat cell differentiation
positive regulation of keratinocyte differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
erythrocyte development
enucleate erythrocyte development
positive regulation of interferon-gamma-mediated signaling pathway
mammary gland branching involved in thelarche
mammary gland branching involved in pregnancy
epithelial cell proliferation involved in mammary gland duct elongation
positive regulation of G0 to G1 transition
ERK1 and ERK2 cascade
regulation of vitamin D receptor signaling pathway
cellular response to epidermal growth factor stimulus
cellular response to steroid hormone stimulus
cellular response to thyroid hormone stimulus
positive regulation of receptor activity
positive regulation of hepatocyte proliferation
regulation of RNA biosynthetic process
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