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Invitrogen
gamma Adducin Polyclonal Antibody
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FIGURE: 1 / 1
gamma Adducin Antibody (PA5-36235) in WB
Western blot analysis of gamma Adducin in Lane 1: BV2 whole cell lysate (40 µg), Lane 2: PC12 whole cell lysate (40 µg), Lane 3: LO2 whole cell lysate (40 µg), Lane 4: A549 whole cell lysate (40 µg), Lane 5: HepG2 whole cell lysate (40 µg). Samples were incubated with gamma Adducin polyclonal antibody (Product # PA5-36235) at a dilution of 1:500.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-36235
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 420-471 of human Adducin gamma
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 82 kDa.
Purity is >95% by SDS-PAGE.
Target Information
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADDG; adducin 3 (gamma); Adducin gamma; Adducin-like protein 70; cytoskeletal protein; Gamma-adducin; potein kinase C binding protein 35H; Protein kinase C-binding protein 35H; unnamed protein product
Gene Aliases: ADD3; ADDL; AI463285; CPSQ3; R75380
UniProt ID: (Mouse) Q9QYB5, (Rat) Q62847
Entrez Gene ID: (Human) 120, (Mouse) 27360, (Rat) 25230
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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