Invitrogen

SIAH1/SIAH2 Polyclonal Antibody

Name: SIAH1\/SIAH2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-36274
Price: 5071.00 CNY
Availability: InStock

查看其他2个SIAH1/SIAH2抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (4)

SIAH1/SIAH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 4

SIAH1/SIAH2 Antibody (PA5-36274) in IHC (P)

Immunohistochemistry analysis of SIAH1/SIAH2 in paraffin-embedded human stomach carcinoma tissue (membrane and cytoplasmic staining) and negative control (right, with PBS only). Samples were incubated with SIAH1/SIAH2 polyclonal antibody (Product # PA5-36274) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

SIAH1/SIAH2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

Immunogen image

Immunogen Image SIAH1/SIAH2 Polyclonal Antibody

产品信息

PA5-36274

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids 178-229 of human Siah-1

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 50% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Ambient (domestic); Wet ice (international)

AB_2553449

产品详细信息

This antibody detects endogenous protein at a molecular weight of 40 kDa.

Purity is >95% by SDS-PAGE.

靶标信息

SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Cite this product

生物信息学

蛋白别名: EC 6.3.2; FLJ08065; hSIAH1; mSiah2; RING-type E3 ubiquitin transferase SIAH1; RING-type E3 ubiquitin transferase SIAH2; seven in absentia 1A; seven in absentia 2; seven in absentia homolog 1; seven in absentia homolog 2; siah-1; siah-1a; siah-2; unnamed protein product

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基因别名: BURHAS; hSiah2; SIAH; SIAH1A; Sinh2