Invitrogen

NEU1 Polyclonal Antibody

Name: NEU1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-42552
Availability: InStock

高级验证

4个已发表图片

5 篇参考文献

查看其他9个NEU1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

NEU1 Antibody in Immunohistochemistry (IHC)

图: 1 / 7

NEU1 Antibody (PA5-42552) in IHC

Immunohistochemistry analysis of human muscle tissue using an anti-NEU1 polyclonal antibody (Product # PA5-42552).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

NEU1 Antibody — Fig 3 The core promoter regions of MSH5 , NEU1 , and PRRC2A are required for optimal POU5F1 expression in hESC (A) The core promoter regions of MSH5 , NEU1 , and PRRC2A were deleted by two sets of distinct sgRNAs (orange bars, Deletion 1 and 2). Mutant cell clones harboring mono-allelic deletions on the P1 allele (green curves), or P2 allele (magenta curves) were identified after genotyping and sequencing of the phased SNPs. FACS analysis was performed for all the mutant clones and wild-type cells (WT: black curves) at day 25 and day 40 after transfection. The FACS data is quantified with FlowJo. P -value is computed using two-sample t-test. (B, C) The H1 POU5F1-eGFP cells were transfected with either control scrambled siRNA or siRNAs targeting the gene as indicated. Each gene is targeted by two sets of siRNAs (SMARTpool and WI design) with different sequences. The cells were analyzed 48 hours after transfection. (B) Whole cell extract was collected and subjected to western blot analysis with indicated antibodies. (C) An aliquot of cells were dissociated into single cells for FACS analysis. Black, magenta, and green curves represent the data from cells treated with Scrambled siRNA (Ctrl), SMARTpool siRNA and WI ( http://sirna.wi.mit.edu/ ) designed siRNA, respectively. {KD}

产品信息

PA5-42552

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

5 µg/mL

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产品规格

种属反应

Human

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the middle region of human NEU1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS with 2% sucrose

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2605865

产品详细信息

Peptide sequence: VWSKDDGVSW STPRNLSLDI GTEVFAPGPG SGIQKQREPR KGRLIVCGHG

Sequence homology: Cow: 100%; Dog: 79%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%

靶标信息

NEU1 is a lysosomal enzyme, which cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A. Mutations in NEU1 gene can lead to sialidosis.The protein encoded by this gene encodes the lysosomal enzyme, which cleaves terminal sialic acid residues from substrates such as glycoproteins and glycolipids. In the lysosome, this enzyme is part of a heterotrimeric complex together with beta-galactosidase and cathepsin A (the latter also referred to as 'protective protein'). Mutations in this gene can lead to sialidosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Acetylneuraminyl hydrolase; EC 3.2.1.18; exo-alpha-sialidase; FLJ93471; G9 sialidase; hydrolytic enzyme of the sialidase family; Lysosomal sialidase; N-acetyl-alpha-neuraminidase 1; NEU1; sialidase 1 (lysosomal sialidase); Sialidase-1; unnamed protein product

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基因别名: NANH; NEU; NEU1; SIAL1

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Entrez Gene ID: (Human) 4758

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功能

exo-alpha-sialidase activity protein binding

参与通路

ganglioside catabolic process oligosaccharide catabolic process