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Invitrogen
NDUFAF2 Polyclonal Antibody
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FIGURE: 1 / 4
NDUFAF2 Antibody (PA5-50452) in ICC/IF
Immunofluorescence analysis of NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NDUFAF2 Polyclonal Antibody (Product # PA5-50452) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-50452
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein corresponding to a region derived from internal residues of human NADH dehydrogenase (ubiquinone) complex I, assembly factor 2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
2.6 mg/mL
Amount
260 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects endogenous levels of total NDUFAF2 protein.
Target Information
Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kappa-B-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B172-like; B172L; mimitin, mitochondrial; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_1; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_3; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 2 isoform NDUFAF2_4; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2; NADH dehydrogenase (ubiquinone) complex I, assembly factor 2; NADH dehydrogenase 1 alpha subcomplex assembly factor 2; NDUFA12-like protein; unnamed protein product
Gene Aliases: B17.2L; MC1DN10; mimitin; MMTN; NDUFA12L
UniProt ID: (Human) Q8N183
Entrez Gene ID: (Human) 91942
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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