Invitrogen

GPR173 Polyclonal Antibody

1个已发表图片

5 篇参考文献

查看其他6个GPR173抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (1)
已发表图片 (1)

GPR173 Antibody in Western Blot (WB)

GPR173 Antibody in Western Blot (WB)

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GPR173 Antibody (PA5-50976) in WB

Western Blot analysis of GPR173 was performed using 3T3 cells. The membrane was probed with GPR173 Polyclonal Antibody (Product # PA5-50976).

GPR173 Antibody in Western Blot (WB)

GPR173 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

GPR173 Antibody in Western Blot (WB) — Western Blot analysis of GPR173 was performed using 3T3 cells. The membrane was probed with GPR173 Polyclonal Antibody (Product # PA5-50976).

GPR173 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF) — Figure 5. Bioinformatics and histology screening for a novel CCK receptor. A , Percentage identity correlation matrix of orphan receptors with CCK1R/2R. B , Left, Illustration of mDlx-DIO-ChR2-mCherry injected into the AC of CCK-Cre mice. Middle, Immunochemistry staining images of candidate receptors in CCK-GABAergic synapses. Scale bars: 5 um. Expanded images are from the white rectangles (top right). The white arrows indicate the colocalization of GPR173 with CCK-GABAergic synapses. Scale bars: 1 um. C , Bar chart shows numbers of overlay dots [bottom right, mCherry + , Syp (synaptophysin) + , and GPCR + , per mm 2 ; n = 3 independent experiments; one-way ANOVA with Tukey's post hoc test, **** p < 0.0001]. D , Confocal images of Flag-GPR173-transfected HEK293T cells colabeled with anti-GPR173, anti-Flag, and DAPI. Scale bar, 10 um. Quantitative analysis of colocalization of Flag and GPR173 across the dotted line (labeled in confocal images). The bar chart depicts the percentage of colabeled cells positive for Flag and GPR173 from Flag-GPR173-transfected HEK293T cells stained with anti-GPR173, anti-Flag, and DAPI ( n = 21 cells). E , Super-resolution image of colocalized GPR173 with CCK-GABAergic synapses, GABA A R with CCK-GABAergic synapses, and GABA A R and GPR173 (left). Scale bar, 1 um. The values for PCC and MOC indicate the colocalization possibility of mCherry with GPR173, mCherry with GABA A R, and GABA A R with GPR173. F , Immunochemistry images of costaining of GP

Immunogen image

GPR173 Polyclonal Antibody

Immunogen Image GPR173 Polyclonal Antibody

产品信息

PA5-50976

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

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免疫组化 (IHC)

-

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免疫组化（冰冻） (IHC (F))

-

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免疫细胞化学 (ICC/IF)

1:200-1:1,000

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Pig

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthesized peptide derived from the Internal region of human GPR173.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 0.5% BSA, 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

GPR173 Polyclonal Antibody detects endogenous levels of GPR173 protein.

靶标信息

This gene encodes a member of the G-protein coupled receptor 1 family. This protein contains 7 transmembrane domains and conserved cysteine residues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: G protein-coupled receptor SREB3; Probable G-protein coupled receptor 173; RP1-290F12.1; Super conserved receptor expressed in brain 3

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基因别名: 3230401K02Rik; GPR173; SREB3

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UniProt ID: (Mouse) Q6PI62, (Rat) Q9JJH2

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Entrez Gene ID: (Mouse) 70771, (Rat) 64021

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signal transducer activity G-protein coupled receptor activity gonadotropin-releasing hormone receptor activity G-protein coupled receptor

signal transduction G-protein coupled receptor signaling pathway cellular response to gonadotropin-releasing hormone negative regulation of neuron migration