Invitrogen

IFI16 Polyclonal Antibody

Name: IFI16 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-51690

高级验证

查看其他17个IFI16抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)
高级验证 (3)

IFI16 Antibody in Immunocytochemistry (ICC/IF)

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IFI16 Antibody (PA5-51690) in ICC/IF

Immunofluorescent staining of IFI16 in human cell line U-251 MG using an IFI16 Polyclonal Antibody (Product # PA5-51690) shows localization to nucleus and nucleoli.

IFI16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IFI16 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IFI16 Antibody — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of IFI16 was observed specifically in SK-MEL-30 cells and in Caco-2 cells using anti-IFI16 Polyclonal Antibody (Product # PA5-51690) in western blot. The relative expression levels of IFI16 within each cell line is shown using RNA-Seq. {RE}

Immunogen image

Immunogen Image IFI16 Polyclonal Antibody

产品信息

PA5-51690

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human IFI16. Recombinant protein control fragment (Product #RP-101035).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2642634

产品详细信息

Immunogen sequence: KEKFTPKKII AIANYVCRNG FLEVYPFTLV ADVNADRNME IPKGLIRSAS VTPKINQLCS QTKGSFVNGV FEVHKKNVRG EFTYYEIQDN TGKMEVVVHG RLTTINCEEG DKLKLTCFEL APKSGNTGEL RSVIHSHIKV IK

Highest antigen sequence identity to the following orthologs: Mouse - 53%, Rat - 39%.

靶标信息

IFI16 (gamma-inteferon-inducible protein 16) binds double-stranded DNA. It binds preferentially to supercoiled DNA and cruciform DNA structures. It seems to be involved in transcriptional regulation and as a transcriptional repressor. IFI16 may have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. It controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. IFI16 is also involved in innate immune response by recognizing viral dsDNA in the cytosol and nucleus. After binding to viral DNA in the cytoplasm, IFI16 recruits TMEM173/STING and mediates the induction of IFN-beta. It is necessary to activate the IFR3 signaling cascade suring human herpes simplex virus 1 infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibiton of viral immediate-early gene expression and replication. IFI16 is also involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Gamma-interferon-inducible protein 16; IFI 16C; Ifi-16; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator

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基因别名: IFI16; IFNGIP1; PYHIN2

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UniProt ID: (Human) Q16666

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Entrez Gene ID: (Human) 3428

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding double-stranded DNA binding protein binding transcription factor binding poly(A) RNA binding DNA-binding transcription factor

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