Invitrogen

DNA2 Polyclonal Antibody

5个已发表图片

5 篇参考文献

查看其他7个DNA2抗体

问题与答案

问题与答案

问题与答案

Additional Information:

{{banner.description}}

{{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}

已发表图片 (5)

DNA2 Antibody in Western Blot (WB)

DNA2 Antibody in Western Blot (WB)

图: 1 / 5

DNA2 Antibody (PA5-68167) in WB

Cancer research communications 2023 - FIGURE 3 d16 displays on-target effects on DNA2 in mutp53-harboring breast and ovarian cancer cells. Cpd d16 modestly induces apoptosis in MDA-MB-468 ( A ) and MDAH-2774 cells ( B ). Cells were treated with DMSO vehicle or d16 (5 or 10 umol/L) for 21 hours. Active caspase-3/7 was determined by Caspase-Glo 3/7 Assay, and was normalized to protein concentrations. The data shown represent the mean +- SD in MDA... View More

DNA2 Antibody in Western Blot (WB)

DNA2 Antibody in Western Blot (WB)

DNA2 Antibody in Western Blot (WB)

DNA2 Antibody in Western Blot (WB)

DNA2 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

DNA2 Antibody in Western Blot (WB) — FIGURE 3 d16 displays on-target effects on DNA2 in mutp53-harboring breast and ovarian cancer cells. Cpd d16 modestly induces apoptosis in MDA-MB-468 ( A ) and MDAH-2774 cells ( B ). Cells were treated with DMSO vehicle or d16 (5 or 10 umol/L) for 21 hours. Active caspase-3/7 was determined by Caspase-Glo 3/7 Assay, and was normalized to protein concentrations. The data shown represent the mean +- SD in MDA-MB-468 ( n = 6 biological replicates) or MDAH-2774 ( n = 3 biological replicates). *, P < 0.05; **, P < 0.01; and ***, P < 0.001 indicating statistically significant difference (two-tailed t test). C and D, Flow cytometric cell cycle analysis of MDAH-2774 cells treated with vehicle (Veh) or 20 umol/L d16 overnight. C, Quantification of cells stained with PI indicates the percentage of cell distribution in G 1 -, S-, and G 2 -M-phase. D, Representative PI profiles. *, P < 0.05; **, P < 0.01 (two-tailed t test, n = 3 biological replicates). E, Colony formation of H1299 stable cells harboring a DNA2 shRNA (shDNA2) or a scrambled shRNA (shScr). Cells were treated with vehicle (Veh) or 2 umol/L d16 for 24 hours and cultured for additional 7 days in a drug-free medium. Bar chart shows the percentage of colonies relative to untreated cells of each group. Error bars = mean +- SD; n = 3; *, P < 0.05. Right: Western blotting confirms DNA2 knockdown in H1299 cells. BT549 ( F ) and MDAH-2774 ( G ) stable cell lines expressing a scrambled shRNA (shScr) or a DNA2 shRNA (shDNA2) were tre

DNA2 Antibody in Western Blot (WB) — FIGURE 1 DNA2 is upregulated in many types of cancer, which is at least in part mediated by mutant p53. A, Expression of DNA2 is significantly elevated in 14 cancers from TCGA database compared with normal tissues. Tumor samples are denoted with bolded lines. Significance is calculated by Mann-Whitney U test. AC, adenocarcinoma; SC, squamous cell carcinoma; CC, clear cell; CS, carcinosarcoma; EC, endometrial carcinoma. B, Expression of DNA2 in breast invasive carcinoma, lung squamous carcinoma, uterine corpus endometrial carcinoma, and ovarian serous carcinoma from TCGA dataset based on TP53 mutation status. The data from other types of cancer are shown in Supplementary Fig. S1 . WT: wild type. C, Depletion of mutp53 decreased DNA2 mRNA expression in MDA-MB-231 and MDA-MB-468 cells. Gene expression data were extracted from GSE26262 () and GSE31812 (), in which gene expression was performed using Affymetrix microarray and normalized using the Robust Multichip Average (RMA) algorithm (). In MDA-MB-468 cells, the short hairpin RNA (shRNA) against mutp53 was induced by doxycycline (Dox). D, DNA2 mRNA and protein expression in p53-null H1299 cells transfected with either an empty vector, WT p53, or indicated mutp53 (V143A, R175H, R248W, R249S, and R273H). Forty-eight hours after transfection, DNA2 mRNA was measured by qRT-PCR and normalized by GAPDH. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 indicating statistically significant difference (two-tailed t test). Right: p53 and DN

DNA2 Antibody in Western Blot (WB) — FIGURE 6 DNA2 inhibitor d16 decreases HR repair. A, Quantification of HR activity in HEK293T cells using the TLR assay after treatment with d16 (20 umol/L). **, P < 0.01 (two-tailed t test). A representative set of flow profiles are shown in Supplementary Fig. S6 . B, MDAH-2774 cells were treated with d16, RU or both for 8 hours. After fixation and permeabilization, immunostaining was performed with an anti-RAD51 rabbit antibody, followed by Texas Red X-conjugated anti-rabbit secondary antibody. Nuclei were stained with Hoechst 33258. Pictures shown are representative images at 100X magnification. C, Quantification of RAD51 foci-positive cells. ***, P < 0.001 (two-tailed t test). D, Immunoblotting shows the expression of DNA2, Rad51, gammaH2AX, or beta-actin in the whole-cell lysates. The Ub-gammaH2AX signals were quantified using NIH ImageJ software.

DNA2 Antibody in Western Blot (WB) — Sex differences in DDR signalling due to BRCA1 heterozygosity 1-week post-rmTBI. Activation of the DDR was assessed using qPCR ( n = 6 per group) and Western blots ( n = 5-8 per group) in males (A,B) and females (C,D) 1-week post-injury. H2AX, p53, EXO1 and RAD51 mRNA expression were measured with qPCR and represented as log 2 fold change (mean +- SEM) (A,C) . Protein expression of yH2AX, pATM, and DNA2 were measured using Western blot (B,D; left) , followed by quantification of peak band intensity and represented as relative expression (mean +- SEM) (B,D; right) . Statistical significance was determined as p < 0.05 using two-way ANOVA to assess main effect of injury (#), genotype (@), or interaction (%), with unpaired t -test used for pairwise comparisons to WT shams (*). Significance levels: p > 0.05 (ns), p < 0.05 (#, @, %, *), p < 0.01 (##, @@, %%, **), p < 0.001 (###, @@@, %%%, ***).

DNA2 Antibody in Western Blot (WB) — Fig. 16 a qPCR and b Western Blot validation. Statistical analysis done in R using unpaired student t-test vs sham

Immunogen image

DNA2 Polyclonal Antibody

Immunogen Image DNA2 Polyclonal Antibody

产品信息

PA5-68167

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

查看5篇已发表文章 5篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthesized peptide derived from human DNA2.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Predicted to react with Mouse samples.

靶标信息

DNA2 is a conserved helicase/nuclease involved in themaintenance of mitochondrial and nuclear DNA stability.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DNA replication ATP-dependent helicase-like homolog; DNA replication ATP-dependent helicase/nuclease DNA2; DNA2-like helicase; hDNA2

查看更多收起

基因别名: DNA2; DNA2L; E130315B21Rik; KIAA0083

查看更多收起

UniProt ID: (Mouse) Q6ZQJ5

查看更多收起

Entrez Gene ID: (Mouse) 327762

查看更多收起

nucleotide binding DNA binding catalytic activity helicase activity nuclease activity endonuclease activity protein binding ATP binding hydrolase activity site-specific endodeoxyribonuclease activity, specific for altered base 5'-flap endonuclease activity phosphoric ester hydrolase activity 5'-3' DNA helicase activity single-stranded DNA-dependent ATPase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity metal ion binding iron-sulfur cluster binding 4 iron, 4 sulfur cluster binding

DNA replication checkpoint DNA double-strand break processing DNA replication mitochondrial DNA replication DNA repair base-excision repair cellular response to DNA damage stimulus metabolic process DNA duplex unwinding DNA replication, Okazaki fragment processing DNA replication, removal of RNA primer mitochondrial DNA repair positive regulation of DNA replication nucleic acid phosphodiester bond hydrolysis