Invitrogen

ATG4B Polyclonal Antibody

Name: ATG4B Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-72646
Availability: InStock

查看其他20个ATG4B抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (2)

图: 1 / 2

ATG4B Antibody (PA5-72646) in WB

Western blot analysis of ATG4B in various lysates. Samples were incubated with ATG4B polyclonal antibody (Product # PA5-72646) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image ATG4B Polyclonal Antibody

产品信息

PA5-72646

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

产品规格

种属反应

Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

200 µg

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2718500

靶标信息

Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

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